FITC标记的胚胎干细胞关键蛋白抗体
产品名称： FITC标记的胚胎干细胞关键蛋白抗体
英文名称： Anti-Oct4/FITC
产品编号： HZ-0830R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： Flow-Cyt=1:50-200 IF=1:50-200
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Rabbit Anti-Oct4/FITC Conjugated antibody
FITC标记的胚胎干细胞关键蛋白抗体
| 英文名称 | Anti-Oct4/FITC |
| 中文名称 | FITC标记的胚胎干细胞关键蛋白抗体 |
| 别 名 | MGC22487; Oct 3; Oct 4; Oct-3; Oct-4; OCT3; Oct4; Octamer binding protein 3; Octamer-binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding transcription factor 3; Octamer-4; POU domain transcription factor OCT4; OTF3; OTF4; OTF 3; OTF 4; OTF-3; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU domain class 5 transcription factor 1; PO5F1_HUMAN; POU type homeodomain containing DNA binding protein ; POU5F1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 染色质和核信号 信号转导 干细胞 细胞周期蛋白 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, |
| 产品应用 | Flow-Cyt=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 39kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Octamer4 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state. Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Subunit: Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Subcellular Location: Cytoplasm.Nucleus.Note=Expressed in a diffuse and slightly punctuate pattern. Tissue Specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Post-translational modifications: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity). Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation (By similarity). ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription. Similarity: Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. Database links: Entrez Gene: 282316 Cow Entrez Gene: 5460 Human Entrez Gene: 18999 Mouse Entrez Gene: 100127461 Pig Entrez Gene: 294562 Rat Omim: 164177 Human SwissProt: O97552 Cow SwissProt: Q01860 Human SwissProt: P20263 Mouse SwissProt: Q9TSV5 Pig Unigene: 249184 Human Unigene: 632482 Human Unigene: 646545 Human Unigene: 17031 Mouse Unigene: 161748 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
POU结构域转录因子OCTAM-4（OCT-4）的表达被广泛认为是多能干细胞的标志。Oct-4与多能干细胞的关系通过它与未分化的多能干细胞紧密限制性表达来体现。在体细胞系分化后，OCT-4的表达迅速消失。与多数多能干细胞标记不同，OCT-4的生物学作用已得到很好的表征。在小鼠中进行的研究指出，10月-4日在未提交状态下建立和/或维持多能干细胞中的关键作用。