Rabbit Anti-EED/FITC Conjugated antibody
|别 名||eed; EED protein; EED_HUMAN; Embryonic ectoderm development; Embryonic ectoderm development isoform a; Embryonic ECTODERM development protein homolog; hEED; OTTHUMP00000235483; OTTHUMP00000235484; OTTHUMP00000235485; Polycomb protein eed; WAIT 1; WAIT-1; WAIT1; WD protein associating with integrin cytoplasmic tails 1.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|交叉反应||Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep, Xenopus laevis, Xenopus tropicalis , Medaka fish|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||50kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human EED|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The transcriptional repressing Polycomb-group (PcG) and transcriptional activating trithorax-group (trxG) genes of Drosophila are part of a cellular memory system responsible for the stable inheritance of gene activity. PcG proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. EED (embryonic ectoderm development) is the human homolog of Eed, a murine PcG gene homologous to the Drosophila homeotic gene, extra sex combs. The human EED protein is 99. 5% identical to the mouse EED protein and contains seven WD repeats, which are involved in protein-protein interactions. There are two human EED transcripts that contain a putative 407-nucleotide-long intron and give rise to two HEED protein isoforms, 535 and 494 amino acids in length. EED interacts in a highly specific manner, both in vitro and in vivo, with histone deacetylase (HDAC) proteins.
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Interacts with MLL (By similarity). Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC, HDAC2, histone H1 and YY1. May interact with ITGA4, ITGAE and ITGB7. May interact with the MA protein of HIV-1.
Nucleus. Chromosome. Transiently colocalizes with XIST at inactive X chromosomes.
Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.
Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.
Belongs to the WD repeat ESC family.
Contains 7 WD repeats.
Entrez Gene: 426381 Chicken
Entrez Gene: 404183 Cow
Entrez Gene: 8726 Human
Entrez Gene: 13626 Mouse
Entrez Gene: 398420 Xenopus laevis
Omim: 605984 Human
SwissProt: Q5ZKH3 Chicken
SwissProt: Q3SZ25 Cow
SwissProt: O75530 Human
SwissProt: Q921E6 Mouse
SwissProt: Q8UUP2 Xenopus laevis
Unigene: 503510 Human
Unigene: 380914 Mouse
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
果蝇转录抑制多梳群（PcG）和转录激活三胸群（trxG）基因是负责基因活性稳定遗传的细胞记忆系统的一部分。PcG蛋白组装成多聚体蛋白复合物，在连续的细胞世代中维持基因的转录抑制状态。胚胎外胚层发育(.nic ectoderm Development，EED)是与果蝇同源基因、超性梳(extra.combs)同源的小鼠PcG基因Eed的人类同源物。人EDE蛋白为99。5%与小鼠EED蛋白相同，包含七个WD重复序列，其涉及蛋白质-蛋白质相互作用。有两种人EED转录本，它们包含一个假定的407核苷酸长的内含子，并产生两个HEED蛋白亚型，长度为535和494个氨基酸。在体外和体内，ED与组蛋白去乙酰化酶（HDAC）蛋白相互作用非常特异。