Rabbit Anti-Epsin 1/FITC Conjugated antibody
|别 名||EH domain binding mitotic phosphoprotein; EPN1; EPN 1; EPN-1; EPS 15 interacting protein 1; EPS-15-interacting protein 1; Epsin 1; EPN1_HUMAN.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||细胞生物 神经生物学 信号转导|
|交叉反应||Human, Mouse, Rat, Dog, Horse, Sheep,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||60kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human Epsin 1/EPN1|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.|
Epsin 1 is an endocytic accessory protein, with significant similarity to the Xenopus mitotic phosphoprotein MP90. Epsin 1 interacts with Eps15 (the ?subunit of the Clathrin adaptor AP2), Clathrin and other accessory proteins. The mitotic phosphorylation of these proteins may be one of the mechanisms by which the invagination of Clathrin-coated pits is blocked in mitosis. Both Epsin and Eps15, like other cytosolic components of the synaptic vesicle endo-cytic machinery, undergo constitutive phosphorylation and depolarization-dependent dephosphorylation in nerve terminals. Epsin 1 also contributes to the mechanism of Clathrin-vesicle-dependent endocytosis. The human Epsin 1 protein contains an Epsin N-terminal homology (ENTH) region and a single Clathrin-binding (LVDLD) motif. Epsin 1 localizes to the leading edge of a vesicular coated pit where the membrane is being actively bent.
Epsin 1 is a cytosolic adaptor protein and interacting partner for the EH domain containing region of Eps15. It is involved in the regulation of clathrin mediated endocytosis via its direct interactions with clathrin, the clathrin adaptor AP-2, and Eps15. Epsin 1 is a cargo specific adaptor for influenza virus entry through the clathrin mediated pathway. In addition to being a clathrin interacting protiein, Epsin 1 is also an ubiquitin, and phospholipid interacting protein. It also may function in a signaling pathway connecting the endocytic machinery to the regulation of nuclear function.
Monomer. Binds clathrin, ZBTB16/ZNF145 and ITSN1. Binds ubiquitinated proteins (By similarity). Binds REPS2, EPS15, AP2A1 and AP2A2. Interacts with RALBP1 in a complex also containing NUMB and TFAP2A during interphase and mitosis. Interacts with AP2B1. [SUBCELLULAR LOCATION]
Cytoplasm (By similarity). Cell membrane; Peripheral membrane protein (By similarity). Nucleus (By similarity). Membrane, clathrin-coated pit (By similarity). Note=Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity).
Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
Ubiquitinated (By similarity).
Belongs to the epsin family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 3 UIM (ubiquitin-interacting motif) repeats.
Entrez Gene: 29924 Human
Entrez Gene: 13854 Mouse
Entrez Gene: 117277 Rat
Omim: 607262 Human
SwissProt: Q9Y6I3 Human
SwissProt: Q80VP1 Mouse
SwissProt: O88339 Rat
Unigene: 279953 Human
Unigene: 3091 Mouse
Unigene: 30007 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
EpSin 1是一种内吞的辅助蛋白，与爪蟾有丝分裂***蛋白MP90有显著的相似性。EpSin 1与EPS15相互作用（？Calthin适配器AP2的亚基、Clathrin和其他辅助蛋白。这些蛋白的有丝分裂***酸化可能是有丝分裂阻断包膜小凹内陷的机制之一。Epsin和Eps15与突触囊泡内吞细胞的其他胞浆成分一样，在神经末梢经历组成性***酸化和去极化依赖性去***酸化。EpSin 1也有助于网格蛋白囊泡依赖的内吞作用机制。人EpSin 1蛋白包含一个EpSN N-末端同源性（THEN）区域和一个单一的网格蛋白结合（LVDLD）基序。EpSin 1定位于囊泡涂覆的坑的前缘，其中膜被主动弯曲。