Rabbit Anti-DDX60/FITC Conjugated antibody
|别 名||DDX60; DDX60_HUMAN; DEAD (Asp-Glu-Ala-Asp) box polypeptide 60; DEAD box protein 60; Probable ATP-dependent RNA helicase DDX60.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||细胞生物 神经生物学 新陈代谢|
|交叉反应||Human, Mouse, Rat,|
|产品应用||Flow-Cyt=3ug/Test ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||198kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human DDX60|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
DDX60 is a 1,712 amino acid protein belonging to the helicase family. DDX60 contains one helicase ATP-binding domain and one helicase C-terminal domain. The gene encoding DDX60 maps to human chromosome 4, which represents approximately 6% of the human genome and contains nearly 900 genes. Notably, the Huntington gene, which is found to encode an expanded glutamine tract in cases of Huntington's disease, is on chromosome 4. FGFR-3 is also encoded on chromosome 4 and has been associated with thanatophoric dwarfism, achondroplasia, Muenke syndrome and bladder cancer. Chromosome 4 is also tied to Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. Chromosome 4 reportedly contains the largest gene deserts (regions of the genome with no protein encoding genes) and has one of the two lowest recombination frequencies of the human chromosomes.
Belongs to the helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Entrez Gene: 55601 Human
Entrez Gene: 234311 Mouse
Entrez Gene: 100360801 Rat
Omim: 613974 Human
SwissProt: Q8IY21 Human
Unigene: 591710 Human
Unigene: 275233 Mouse
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit Anti-DDX60 antibody (bs-14253R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
DDX60是属于解旋酶家族的1712氨基酸。DDX60包含一个解旋酶ATP结合结构域和一个解旋酶C-末端结构域。编码DDX60的基因定位于人类第4染色体，它代表人类基因组的大约6%，包含近900个基因。值得注意的是，亨廷顿基因位于第4染色体上，在亨廷顿病的病例中发现它编码一个扩张的谷氨酰胺束。FGFR-3还编码在第4号染色体上，并与丘脑侏儒症、软骨发育不良、Muenke综合征和膀胱癌有关。第4号染色体也与Ellis van Creveld综合征、甲基丙二酸血症和多囊肾病有关。据报道，4号染色体含有最大的基因沙漠（基因组中没有蛋白质编码基因的区域），并具有人类染色体的两个最低重组频率之一。