Rabbit Anti-phospho-Dematin (Ser403)/FITC Conjugated antibody
|别 名||Dematin (phospho S403); P-Dematin (phospho S403); DEMA; DEMA_HUMAN; Dematin; DMT; EPB49; erythrocyte membrane protein band 4.9 (dematin); Erythrocyte membrane protein band 4.9.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||细胞生物 信号转导 结合蛋白 细胞骨架|
|交叉反应||Human, Mouse, Rat, Pig, Cow, Sheep,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||45kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated Synthesised phosphopeptide derived from human Dematin around the phosphorylation site of Ser403|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The protein encoded by this gene is an actin binding and bundling protein that plays a structural role in erythrocytes, by stabilizing and attaching the spectrin/actin cytoskeleton to the erythrocyte membrane in a phosphorylation-dependent manner. This protein contains a core domain in the N-terminus, and a headpiece domain in the C-terminus that binds F-actin. When purified from erythrocytes, this protein exists as a trimer composed of two 48 kDa polypeptides and a 52 kDa polypeptide. The different subunits arise from alternative splicing in the 3' coding region, where the headpiece domain is located. Disruption of this gene has been correlated with the autosomal dominant Marie Unna hereditary hypotrichosis disease, while loss of heterozygosity of this gene is thought to play a role in prostate cancer progression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]
Membrane-cytoskeleton-associated protein with F-actin-binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphorylation by the cAMP-dependent protein kinase A (PKA). Binds to the erythrocyte membrane glucose transporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches the spectrin-actin network to the erythrocytic plasma membrane. Plays a role in maintaining the functional integrity of PKA-activated erythrocyte shape and the membrane mechanical properties. Plays also a role as a modulator of actin dynamics in fibroblasts; acts as a negative regulator of the RhoA activation pathway. In platelets, functions as a regulator of internal calcium mobilization across the dense tubular system that affects platelet granule secretion pathways and aggregation. Also required for the formation of a diverse set of cell protrusions, such as filopodia and lamellipodia, necessary for platelet cell spreading, motility and migration. Acts as a tumor suppressor and inhibits malignant cell transformation.
Monomeric (isoform 2); under reducing conditions. Self-associates. Exists under oxidizing condition as a trimer of two isoforms 2 and isoform 1 linked by disulfide bonds (Probable). Found in a complex with DMTN, F-actin and spectrin. Found in a complex with ADD2, DMTN and SLC2A1. Interacts with F-actin, ITPKB, RASGRF2 and spectrin. Isoform 2 interacts with SLC2A1 (via C-terminus cytoplasmic region). Isoform 1 and isoform 2 interact (phosphorylated form) with plasmodium berghei 14-3-3 protein; the interaction occurs in a PKA-dependent manner.
Cytoplasm. Cytoplasm, cytosol. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Cell membrane. Membrane. Endomembrane system. Cell projection. Note=Localized at the spectrin-actin junction of erythrocyte plasma membrane. Localized to intracellular membranes and the cytoskeletal network. Localized at intracellular membrane-bounded organelle compartment in platelets that likely represent the dense tubular network membrane. Detected at the cell membrane and at the parasitophorous vacuol in malaria-infected erythrocytes at late stages of plasmodium berghei or falciparum development.
Expressed in platelets (at protein level). Expressed in heart, brain, lung, skeletal muscle, and kidney.
Phosphorylated. Phosphorylation at Ser-403 by PKA causes the C-terminal headpiece domain to associate with the N-terminal core domain, and leads to the inhibition of its actin bundling activity.
The N-terminus is blocked.
Belongs to the villin/gelsolin family.
Contains 1 HP (headpiece) domain.
Entrez Gene: 2039 Human
Entrez Gene: 13829 Mouse
Entrez Gene: 361069 Rat
Omim: 125305 Human
SwissProt: Q08495 Human
SwissProt: Q9WV69 Mouse
Unigene: 106124 Human
Unigene: 210863 Mouse
Unigene: 446654 Mouse
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
该基因编码的蛋白是一种肌动蛋白结合和捆绑蛋白，通过以***酸化依赖的方式稳定和将波谱蛋白/肌动蛋白细胞骨架附着到红细胞膜上，在红细胞中发挥结构作用。这种蛋白质包含在N-末端的核心结构域，以及结合F-肌动蛋白的C-末端中的头部结构域。当从红细胞纯化时，该蛋白质作为由两个48 kDa多肽和52 kDa多肽组成的三聚体存在。不同亚基起源于3′编码区的选择性剪接，其中头部域位于其中。该基因的破坏与常染色体显性遗传性玛丽·昂纳氏低血压病相关，而该基因杂合性的丢失被认为在前列腺癌进展中起作用。选择性剪接导致多个转录子变体编码不同的亚型。[ RefSeq，2014月11日]