Anti-phospho-EIF4B (Ser406)抗体上海钰博生物科技有限公司

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产品名称: Anti-phospho-EIF4B (Ser406)抗体
英文名称: phospho-EIF4B (Ser406)
抗体货号: YB--14551R
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 1580元
抗原: KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser406:HP(p-S)WR
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/10/11 12:58:00
详细资料:  实验方法技术资料
浏览人数:15
诚信指数:1186点
了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Anti-phospho-EIF4B (Ser406)抗体

产品编号 YB-14551R
英文名称 phospho-EIF4B (Ser406)
中文名称 ***酸化真核翻译起始因子4B抗体
别    名 eIF4B (phospho S406); p-eIF4B (phospho S406); EIF 4B; EIF-4B; Eukaryotic initiation factor 4B; IF4B_HUMAN.  
规格价格 100ul/1580元 购买        大包装/询价
说 明 书 100ul  
产品类型 ***酸化抗体 
研究领域 转运蛋白  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Sheep, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 69kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser406:HP(p-S)WR 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot). 

Function:
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Post-translational modifications:
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.

Similarity:
Contains 1 RRM (RNA recognition motif) domain.

SWISS:
P23588

Gene ID:
1975

Database links:

Entrez Gene: 1975 Human

Entrez Gene: 75705 Mouse

Entrez Gene: 300253 Rat

Omim: 603928 Human

SwissProt: P23588 Human

SwissProt: Q8BGD9 Mouse

Unigene: 648394 Human

Unigene: 702041 Human

Unigene: 290022 Mouse

Unigene: 391795 Mouse

Unigene: 95954 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD
Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD
Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(bs-14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-EIF4B (Ser406)) Polyclonal Antibody, Unconjugated (bs-14551R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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