Anti-MCL1/BCL2L3抗体上海钰博生物科技有限公司

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产品名称: Anti-MCL1/BCL2L3抗体
英文名称: MCL1/BCL2L3
抗体货号: YB--20718R
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 1380元
抗原: KLH conjugated synthetic peptide derived from mouse MCL1/BCL2L3:101-200/331
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Mouse, Rat,
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/10/11 14:08:00
详细资料:  实验方法技术资料
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了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Anti-MCL1/BCL2L3抗体

产品编号 YB-20718R
英文名称 MCL1/BCL2L3
中文名称 髓样细胞白血病-1抗体
别    名 myeloid cell leukemia 1; myeloid cell leukemia sequence 1; MCL-1; MCL1L; MCL 1; mcl1/EAT; MGC104264; MGC1839; TM; MCL1S; EAT) Bcl 2 related protein EAT/mcl1; BCL2 related; BCL2L3; EAT; Induced myeloid leukemia cell differentiation protein Mcl 1; myeloid cell leukemia sequence 1; Myeloid cell leukemia sequence 1 BCL2 related; Myeloid cell leukemia sequence 1 isoform 1; OTTHUMP00000032794; OTTHUMP00000032795; TM; MCL1; bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 肿瘤  免疫学  信号转导  细胞凋亡  转录调节因子  线粒体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse, Rat, 
产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 39kDa
细胞定位 细胞核 细胞浆 线粒体
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse MCL1/BCL2L3:101-200/331 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Mcl1 is an anti-apoptotic member of Bcl2 family originally isolated from the ML1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Mcl1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl2 family members, and inhibits apoptosis by a number of cytotoxic stimuli. It is involved in programing of differentiation and concomitant maintenance of viability but not of proliferation. Isoform 1 inhibits apoptosis while isoform 2 promotes it. Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML1.

Function:
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

Subunit:
Interacts with BAD, BOK, BIK and BFM (By similarity). Interacts with PMAIP1. Isoform 1 interacts with BAX, BAK1, TPT1 and BCL2L11. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptotic BCL2-related proteins.

Subcellular Location:
Membrane; Single-pass membrane protein (Potential). Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Note=Cytoplasmic, associated with mitochondria.

Post-translational modifications:
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment. 
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region. 
Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.

Similarity:
Belongs to the Bcl-2 family.

SWISS:
Q07820

Gene ID:
17210

Database links:

Entrez Gene: 4170 Human

Entrez Gene: 17210 Mouse

Entrez Gene: 60430 Rat

Omim: 159552 Human

SwissProt: Q07820 Human

SwissProt: P97287 Mouse

SwissProt: Q9Z1P3 Rat

Unigene: 632486 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

Mcl1蛋白是bcl-2家族成员,它在调控肿瘤细胞的凋亡、增殖、分化等过程中起一定作用。
产品图片
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MCL1) Polyclonal Antibody, Unconjugated (bs-20718R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MCL1) Polyclonal Antibody, Unconjugated (bs-20718R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MCL1) Polyclonal Antibody, Unconjugated (bs-20718R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control (blue line): HepG2 (Black). Primary Antibody (green line): Rabbit Anti-MCL1/BCL2L3 antibody (bs-20718R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The Cells stained with Primary Antibody for 30 min at room temperature.The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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