Anti-ADRA2抗体上海钰博生物科技有限公司

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产品名称: Anti-ADRA2抗体
英文名称: ADRA2
抗体货号: YB--1062R
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 1380元
抗原: KLH conjugated synthetic peptide derived from human ADRA2:385-450/450
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Human, Mouse, Rat, Pig, Cow, Rabbit,
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/12/17 16:48:00
详细资料:  实验方法技术资料
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诚信指数:1476点
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Anti-ADRA2抗体

产品编号 YB-1062R
英文名称 ADRA2
中文名称 ADRA2***能受体2抗体
别    名 alpha-2A adrenergic receptor; alpha 2a Adrenergic Receptor; ADRA2A; ADRA2; ADRA2R; ADRAR; ALPHA2AAR; ZNF32; RATRG20; RG20; Adrenergic alpha 2A receptor; Alpha 2AAR subtype C10; OTTHUMP00000058849; ADA2A_HUMAN; Alpha-2A adrenergic receptor; Alpha-2 adrenergic receptor subtype C10; Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2AAR.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 神经生物学  生长因子和***  细胞膜受体  G蛋白偶联受体  G蛋白信号  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Rabbit, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 50kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ADRA2:385-450/450 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha 2A, alpha 2B, and alpha 2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha 2A and alpha 2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha 2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha 2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha 2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq].

Function:
Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-***. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = ***.

Subcellular Location:
Cell membrane.

Similarity:
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily.
ADRA2A sub-subfamily.

SWISS:
P08913

Gene ID:
150

Database links:

Entrez Gene: 150 Human

Entrez Gene: 11551 Mouse

Entrez Gene: 25083 Rat

Entrez Gene: 266750 Zebrafish

Omim: 104210 Human

SwissProt: P08913 Human

SwissProt: Q01338 Mouse

SwissProt: P22909 Rat

SwissProt: Q1L8Y8 Zebrafish

SwissProt: Q90WY4 Zebrafish

Unigene: 249159 Human

Unigene: 235195 Mouse

Unigene: 170171 Rat

Unigene: 30878 Zebrafish



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Sample: 
Kidney (Rat) Lysate at 30 ug
Brain (Rat) Lysate at 30 ug
Primary: Anti- ADRA2 (bs-1062R) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADRA2) Polyclonal Antibody, Unconjugated (bs-1062R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
 
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-ADRA2 Polyclonal Antibody, Unconjugated(bs-1062R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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