FITC标记的多聚嘧啶序列结合蛋白相关剪接因子PSF抗体
产品名称： FITC标记的多聚嘧啶序列结合蛋白相关剪接因子PSF抗体
英文名称： Anti-SFPQ/FITC
产品编号： HZ-19683R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
- 联系人 : 鲍丽雯
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- 邮编 : 200612
- 所在区域 : 上海
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Rabbit Anti-SFPQ/FITC Conjugated antibody
FITC标记的多聚嘧啶序列结合蛋白相关剪接因子PSF抗体
| 英文名称 | Anti-SFPQ/FITC |
| 中文名称 | FITC标记的多聚嘧啶序列结合蛋白相关剪接因子PSF抗体 |
| 别 名 | 100 kDa DNA pairing protein; 100 kDa DNA-pairing protein; 100 kDa subunit; DNA binding p52/p100 complex 100 kDa subunit; DNA-binding p52/p100 complex; hPOMp100; Polypyrimidine tract binding protein associated splicing factor; Polypyrimidine tract-binding protein-associated-splicing factor; POMP100; proline- and glutamine-rich; PSF; PTB associated splicing factor; PTB-associated-splicing factor; Sfpq; SFPQ_HUMAN; Splicing factor; Splicing factor proline and glutamine rich; Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated). |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 76kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SFPQ |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | Function: DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity. Subcellular Location: Nucleus matrix. Predominantly in nuclear matrix. Post-translational modifications: The N-terminus is blocked. Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA. Arg-7, Arg-9, Arg-19 and Arg-25 are dimethylated, probably to asymmetric dimethylarginine. DISEASE: Note=A chromosomal aberration involving SFPQ may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;1)(p11.2;p34) with TFE3. Similarity: Contains 2 RRM (RNA recognition motif) domains. Database links: Entrez Gene: 6421 Human Entrez Gene: 71514 Mouse Entrez Gene: 252855 Rat Omim: 605199 Human SwissProt: P23246 Human SwissProt: Q8VIJ6 Mouse Unigene: 355934 Human Unigene: 257276 Mouse Unigene: 482296 Mouse Unigene: 54645 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
DNA和RNA结合蛋白，参与了几个核过程。基本的前mRNA剪接因子在剪接体形成早期和剪接催化步骤II中是必需的，可能是与NONO的异构体。结合剪接体C复合物中的前mRNA，并特异性结合内含子聚吡咯束。与U5 snRNA相互作用，可能通过与位于U5 snRNA茎1b3′侧的富含嘌呤的序列结合。可能作为与SNRPA/U1A的无snRNP复合物的组成部分参与前mRNA偶联的剪接和聚腺苷酸化过程。缺陷RNA在核保持中的作用SFPQ可能参与同源DNA配对；在体外，促进ssDNA在双链DNA之间的侵袭并产生D-环形成。SFPQ-NO异构体可能通过调节拓扑异构酶I/TOP1的功能参与DNA解旋，在体外，刺激TOP1与DNA解离，促进其在不同DNA螺旋之间的跳跃。SFPQ-NO异构体可能参与双链断裂修复和V(D)J重组所需的DNA非同源末端连接(NHEJ)，并且可能稳定配对的DNA末端；在体外，该复合物强烈刺激DNA末端连接，直接与DNA底物结合，并与Ku70/G22P1-Ku80/XRcc5（Ku）二聚体建立功能性连接复合物。SFPQ参与转录调控。转录抑制可能通过SFPQ和SIN3A的相互作用以及随后组蛋白去乙酰化酶（HDACs）的募集来介导。SFPQ-NONO/SF-1复合物与CYP17启动子结合，并调节基础和cAMP依赖的转录活性。SFPQ亚型Long与核激素受体的DNA结合结构域（DBD）结合，如RXRA和可能是THRA，在没有激素配体的情况下作为转录辅阻遏剂发挥作用。结合胰岛素样生长因子反应元件（IGFRE）中的DNA序列5’-CTGAGTC-3’，并抑制IGF-I刺激的转录活性。