Rabbit Anti-Intersectin 2/FITC Conjugated antibody
|别 名||Intersectin-2; ITSN2; ITSN2_HUMAN; KIAA1256; PRO2015; SH3 domain-containing protein 1B; SH3D1B; SH3P18; SH3P18-like WASP-associated protein; SWA; SWAP.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||细胞生物 神经生物学 信号转导 G蛋白信号|
|交叉反应||Human, Mouse, Rat,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||193kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human Intersectin 2|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Intersectin, which is also designated Ese1 or ITSN1, is a component of the cellular endocytic machinery. Intersectin is composed of two N-terminal Ese15 homology (EH) domains, a central highly charged region and five C-terminal SH3 domains, which all largely contribute to the association of Intersectin with other components of the endocytic pathway. The EH domain is particularly responsible for the directed localization of Intersectin to Clathrin-coated pits near the plasma membrane. Within the endocytic vesicles the SH3 domains facilitate the binding of Intersectin with Dynamin, and the central domain is essential for the association of Intersectin with SNAP 25. Two isoforms of Intersectin are produced as a result of alternative splicing in a stop codon, and they are designated as Intersectin-short and long (or IntersectinS and IntersectinL) to reflect an extended C-terminal domain. The long form, which has an extended C-terminal domain, is specifically expressed in neurons; the short form is detected in both glial and nonneuronal cells. The related proteins Intersectin-2 and the murine homolog Ese2 also contain the characteristic N-terminal EH domains, the central coiled-coil domain and five C-terminal SH3 domains and are likely involved the endocytic scaffolding complexes.
Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles.
Belongs to a complex that may contain multimers of ITSN1, ITSN2 and EPS15, and different partners according to the step in the endocytic process. Interacts with ADAM15. Interacts with FASLG. Interacts with ANKRD54. Interacts with FCHO2.
Ubiquitous. Isoform 1 is primarily expressed in adult heart and liver.
Contains 1 C2 domain.
Contains 1 DH (DBL-homology) domain.
Contains 2 EF-hand domains.
Contains 2 EH domains.
Contains 1 PH domain.
Contains 5 SH3 domains.
Entrez Gene: 50618 Human
Omim: 604464 Human
SwissProt: Q9NZM3 Human
Unigene: 432562 Human
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic application
交联蛋白，也称为Ese1或ITSN1，是细胞内吞机制的组成部分。交联蛋白由两个N-末端Ese15同源性(EH)结构域、一个中心高电荷区和五个C-末端SH3结构域组成，这些结构域都极大地促进了交联蛋白与内吞途径其他成分的结合。EH结构域特别负责将交联蛋白定向定位于质膜附近的包覆有Clathrin的凹坑。在内吞泡中，SH3结构域促进交联蛋白与动力蛋白的结合，并且中心结构域对于交联蛋白与SNAP 25的结合是必不可少的。由于终止密码子中的选择性剪接，产生了两种形式的Intersectin，它们被指定为Intersectin-.and long(或IntersectinS和IntersectinL)，以反映扩展的C端结构域。具有延伸的C端结构域的长形式在神经元中特异表达，短形式在胶质细胞和非神经元细胞中均可检测到。相关蛋白Intersectin-2和小鼠同源物Ese2还含有特征性的N-末端EH结构域、中心螺旋结构域和五个C-末端SH3结构域，可能与内吞支架复合物有关。