Product Name: SARS-CoV-2(Omicron) Spike S1 Protein（His Tag）
Cat No. : nCoV-S1-Omicron
Description: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic
antigen-related cell adhesion molecule 1; Sia, sialic acid; 0-ac Sia, 0-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits,
S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses,
as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion;Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD),leading to a higher mortality rate.
Protein Construction: A DNA sequence encoding the SARS -CoV-2 Spike S1 (YP_ 009724390.1,with mutations P9L, C136F, 144-145 deletion, R190S, D215G, Y449H, E484K,
N501Y, D614G, H655Y, N679K) (Met1 -Arg685) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1 .529) which emerged in the South Africa
Expression Host:HEK293 Cells
Purity:>90% as determined by SDS-PAGE
Molecular Mass: The recombinant SARS-CoV-2 Spike S1 consists of 678 amino acids and predicts a molecular mass of 76.48 kDa. As a result of glycosylation, it migrates as an approximately 99.56 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as solution form in PBS, pH7.5 or lyophilized from PBS, pH7.5
Shipping:In general, proteins are shipped out with blue ice unless customers require otherwise.
Stability &Storage:Use a manual defrost freezer and avoid
repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
Note:For research use only .Not for use in clinical diagnostic procedures.
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