性能参数
产品名称： IDH2(R172K) 小鼠单抗
英文名称： Anti-IDH2(R172K) Mouse Monoclonal Antibody
抗体货号： 26163
产品规格： 100 微升
级 别： , ,
产品产地： 美国
品牌商标： Neweast Biosciences
价 格： 4800元
抗原来源：
抗体来源： Mouse
适用物种：
应用范围： ELISA, WB, IHC,IF
更新时间： 2023/3/30 9:13:00
详细资料：
实验方法技术资料
浏览人数：198
供应商： 武汉费斯德生物科技有限公司
诚信指数：7点
了解更多：进入公司展台
使用范围：仅限科研使用，不能应用于临床
产品详细描述
| 货号: 26163 |
| 基因符号: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
| 描述: Anti-IDH2(R172K) Mouse Monoclonal Antibody |
| 背景介绍: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas. |
| 免疫原: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IHC: 1: 50-1: 100 IHC: 1: 10-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: recognizes R172K mutant, but not wild-type IDH2 of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:  Western blot analysis of recombinant IDH2(R172K) and wildtype proteins. Purified His-tagged IDH2(R172K) (lane 2) and wildtype protein (lane 1) were blotted with Anti-IDH2(R172K) monoclonal antibody (Cat. #26163). 免疫荧光:   Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172K) antibody. HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid or pCDNA3-GFP- IDH2 (R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (Cat. #26163). |
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