Anti-Human IL-17 (IL-17A)-抗体-抗体-生物在线
上海研卉生物科技有限公司
Anti-Human IL-17 (IL-17A)

Anti-Human IL-17 (IL-17A)

商家询价

产品名称： Anti-Human IL-17 (IL-17A)

英文名称： Anti-Human IL-17 (IL-17A)

产品编号： 500-P07

产品价格： null

产品产地： USA

品牌商标： PEPROTECH

更新时间： null

使用范围： ELISA/WB/Immunohistochemistry/Neutralization

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产品编号:
500-P07
来源:
Polyclonal Rabbit
制备:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17A. Anti-Human IL-17A specific antibody was purified by affinity chromatography employing immobilized hIL-17 matrix.
免疫原:
E.coli derived Recombinant Human IL-17 (IL-17A) (PeproTech catalog #200-17)
应用:
ELISA
Sandwich
To detect hIL-17A by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-17A (500-P07Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17A.
Western Blot
To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralization
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/ml), a concentration of 0.9-1.3 μg/ml of this antibody is required.
Immunohistochemistry
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.