货号：RD001-07规格: 0.1 mg (A280 测定， 与BSA的相关系数为0.704)
溶解: 加0.1ml去离子水，室温下彻底溶解，最终浓度为1 mg/ml。若有轻微混浊，不影响抗体活性，使用前离心即可。
应用： ELISA、Western blotting。
Rabbit Anti-human Adiponectin IgG, PAb
Cat. No.: RD001-07
Size: 0.1 mg (determined by absorbance of A280 and the correlation coefficient against BSA is 0.704).
Antigen: Recombinant human adiponectin produced in HEK293 (Human embryonic kidney cell line). The recombinant human adiponectin is 100% homologous with the human serum adiponectin.
Titer: Is defined by indirect ELISA, it is >1:100,000 for antibody concentration 1mg/ml, 2.5 ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve.
Purification Method: Immunoaffinity chromatography on a column with immobilized human recombinant adiponectin.
Formulation: Sterile filtered and lyophilized from 1 mg/ml in 0.1 M phosphate buffer, 0.1 M NaCl, pH 7.2.
Reconstitution: Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Storage: Store lyophilized antibody at 2-8°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
Stability/Shelf Life: The lyophilized antibody remains stable and fully active until the expiry date when stored at 4°C.
Applications: ELISA, Western blotting.