Cat code 200321
Species Cross-reactivity H,M
Key Application WB,IP,ICC
Antibody type Monoclonal antibody
Purified method Affinity purified
Molecular Weight 300kDa
Sensitivity This antibody detects endogenous levels of MAP2 and does not cross-react with related proteins.
Immunogen Purified recombinant MAP2 protein fragments expressed in E.coli
UniProt Number P11137
GeneBank ID NM_031847
Subcellular location Cytoplasm >cytoskeleton
Formulation Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150mM NaCl)with 0.2%sodium azide,0.1%BSA,50%glycerol.
Altername MAP2A; MAP2B; MAP2C; DKFZp686I2148;MAP2
Gene_symbol MAP2A; MAP2B; MAP2C
Summary This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
UniPort summary Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization.They also seem to have a stiffening effect on microtubules.
Application Images :
Western blot detection of MAP2(N-terminus) antibody in Mouse Brain tissue&Hela cell lysates using MAP2(N-terminus) antibody (1:1000 diluted). Predicted band size: 202KDa Observed band size: 300KDa
Immunocytochemistry of HeLa cells using anti- MAP2 (N-terminus) antibody diluted 1:150.
Immunoprecipitation analysis of Hela cell lysates using MAP2 antibody