Cat code 220034
Species Cross-reactivity H, R, M
Key Application WB
Clone Number 5H7-D5-E2
Antibody type Monoclonal antibody
Molecular Weight 30kDa
Sensitivity The antibody detects endogenous level of PHB protein.
Immunogen Purified recombinant fragment of human PHB expressed in E. Coli.
UniProt Number P35232
GeneBank ID NM_002634.2
Subcellular location Mitochondrion inner membrane
Formulation Ascitic fluid containing 0.03% sodium azide.
Altername PHB; PHB_HUMAN; PHB1; Prohibitin;
Summary This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3´ UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniPort summary Function Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Application Images :
Western blot detection of PHB antibody in C6, COS7, 3T3 and Hela cell lysates using PHB antibody (dilution 1:1000).Predicted band size:30 KDa.Observed band size:30KDa.