Anti-SNAIL+SLUG抗体-抗体-抗体-生物在线
Anti-SNAIL+SLUG抗体

Anti-SNAIL+SLUG抗体

商家询价

产品名称: Anti-SNAIL+SLUG抗体

英文名称: Anti-SNAIL+SLUG抗体

产品编号: ab180714

产品价格: null

产品产地: 英国

品牌商标: abcam

更新时间: null

使用范围:

深圳市宇德立生物科技有限公司
  • 联系人 :
  • 地址 : 深圳市宝安区西乡宝民二路贤基大厦4E
  • 邮编 :
  • 所在区域 : 广东
  • 电话 : 133****4454
  • 传真 : 0755-26457513
  • 邮箱 : 1484332550@qq.com

 

  • 经测试应用IHC-FrWBICC/IFIHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human SNAIL+SLUG aa 236-264. The exact sequence is proprietary.
    Database link: O95863

  • 阳性对照
    • Mouse heart tissue extracts

性能

应用

Our Abpromise guarantee covers the use of ab180714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Abreviews 说明
IHC-Fr   1/100.
WB   1/500 - 1/2000. Predicted molecular weight: 29 kDa.
ICC/IF   1/50 - 1/200.
IHC-P   1/50 - 1/200.

Anti-SNAIL+SLUG antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 200X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 400X.

  • ab180714 staining SNAIL in human MCF10A cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 3.7% PFA in PBS for 15 minutes and blocked with 0.1% Milk + 0.1% Tween in PBS for 30 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in PBS + 0.1% Tween) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab180714 staining SNAIL in mouse embryonic heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100 in blocking buffer) for 16 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-SNAIL+SLUG antibody (ab180714) at 1/500 dilution + Mouse heart tissue extracts

    Predicted band size : 29 kDa


  • Predicted band size : 29 kDa

    Lane 1: Snail recombinant 0.1 ug (55 kDa)

    Lane 2: Slug recombinant 0.1 ug  (56 kDa)