• 经测试应用WB, IHC-P, ICC/IF, Flow Cytmore details
• 种属反应性
  与反应: Mouse, Rat, Chicken, Cow, Dog, Human, Monkey
• 免疫原

  Recombinant fragment within Mouse GAPDH aa 1-150. The exact sequence is proprietary.
  Database link: P16858

  Run BLAST with Run BLAST with 
• 阳性对照
  • WB: HeLa, MDBK, COS-1, MDCK, UMNSAH/DF-1, Jurkat, C6 and NIH/3T3 whole cell lysates; Mouse brain and heart lysates; Rat brain, heart, kidney and spleen lysates; Human fetal brain, heart and kidney lysates. IHC-P: Human transitional cell carcinoma of bladder, Mouse spleen and Rat spleen tissues. ICC/IF: HeLa cells. Flow: Jurkat cells.
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Alternative versions available:

  Anti-GAPDH antibody (Alexa Fluor^® 488) [EPR16884] (ab204276)

  Anti-GAPDH antibody (Alexa Fluor^® 647) [EPR16884] (ab204480)

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
• 浓度100 µl 浓度为 1.485 mg/ml 40 µl 浓度为 1.485 mg/ml 10 µl 浓度为 1.485 mg/ml 
  
• 纯度Protein A purified
• 克隆单克隆
• 克隆编号EPR16884
• 同种型IgG
• 研究领域
  • Neuroscience
   
  • Neurology process
   
  • Neurodegenerative disease
  • Huntington's disease

  • Neuroscience
   
  • Neurology process
   
  • Neurodegenerative disease
  • Alzheimer's disease
   
  • Other

  • Isotype/Loading Controls
   
  • Loading Controls
   
  • GAPDH

  • Signal Transduction
   
  • Metabolism
   
  • Energy Metabolism

  • Cancer
   
  • Cancer Metabolism
   
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolic signaling pathways
  • Energy transfer pathways
   
  • Energy Metabolism

  • Metabolism
   
  • Types of disease
   
  • Neurodegenerative disease

应用

靶标

• 功能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase i