PCNA抗体Applications
| In-house applications |
ELISA, IF, IP, WB, FC, IHC Application not tested? Please see our Guarantee. |
| Published applications |
IF, IHC, WB |
Publications |
50 citations –view here |
| Species specificity |
Human,Mouse,Rat,Zebrafish; other species not tested. |
| Published Species |
Human, mouse, rat, rat; human |
|
Images
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 WB result of PCNA antibody (10205-2-AP, 1:10000) with si-control and si-PCNA transfected HEK293 cells. MCF7 cells were subjected to SDS PAGE followed by western blot with 10205-2-AP(PCNA antibody) at dilution of 1:2000 IP Result of anti-PCNA (IP:10205-2-AP, 3ug; Detection:10205-2-AP 1:1500) with MCF-7 cells lysate 2500ug. Immunohistochemical of paraffin-embedded human stomach cancer using 10205-2-AP(PCNA antibody) at dilution of 1:500 (under 40x lens) Immunofluorescent analysis of HepG2 cells, using PCNA antibody 10205-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). 1X10^6 HEK-293T cells were stained with .2ug PCNA antibody (10205-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
|
| Positive WB detected in |
MCF7 cells, A431 cells, HEK293 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse liver tissue, mouse testis tissue, NIH/3T3 cells, Raji cells, rat liver tissue, rat testis tissue |
| Positive IP detected in |
MCF-7 cells |
| Positive IHC detected in |
Human stomach cancer tissue, human breast cancer tissue |
| Positive IF detected in |
HepG2 cells |
| Positive FC detected in |
HEK-293T cells |
| Recommended dilution |
WB : 1:1000-1:10000
IP : 1:500-1:5000
IHC : 1:200-1:1000
IF : 1:20-1:200 |
PCNA抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information PCNA抗体
| Immunogen |
PCNA fusion protein ag0277 |
Full name |
Proliferating cell nuclear antigen |
Calculated molecular
weight |
29 kDa/31 kDa |
Observed molecular
weight |
36-38 kDa |
GenBank accession
number |
BC000491 |
Gene ID (NCBI) |
5111 |
| Gene symbol |
PCNA |
Synonyms |
PCNA |
Background
|
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. PCNA抗体
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