PFKP 抗体Applications
| In-house applications |
ELISA, WB, IHC, IF, IP Application not tested? Please see our Guarantee. |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
|
Images
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 mouse heart tissue were subjected to SDS PAGE followed by western blot with 13389-1-AP(PFKP antibody) at dilution of 1:1500 IP Result of anti-PFKP (IP:13389-1-AP, 4ug; Detection:13389-1-AP 1:1000) with mouse heart tissue lysate 4000ug. Immunohistochemistry of paraffin-embedded human spleen tissue slide using 13389-1-AP( PFKP Antibody) at dilution of 1:50 (under 10x lens) Immunohistochemistry of paraffin-embedded human spleen tissue slide using 13389-1-AP( PFKP Antibody) at dilution of 1:50 (under 40x lens) Immunofluorescent analysis of BxPC-3 cells using 13389-1-AP( PFKP Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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| Positive WB detected in |
Mouse heart tissue, BxPC-3 cells, HEK-293 cells, HeLa cells, human heart tissue, Jurkat cells, mouse skeletal muscle tissue |
| Positive IP detected in |
Mouse heart tissue |
| Positive IHC detected in |
Human spleen tissue, human heart tissue, human lung cancer tissue |
| Positive IF detected in |
BxPC-3 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100 |
PFKP 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
PFKP fusion protein ag4047 |
Full name |
Phosphofructokinase, platelet |
Calculated molecular
weight |
784aa,86 kDa |
Observed molecular
weight |
86 kDa |
GenBank accession
number |
BC029138 |
Gene ID (NCBI) |
5214 |
| Gene symbol |
PFKP |
Synonyms |
6 phosphofructokinase type C, FLJ40226, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase |
Background PFKP 抗体
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PFKP(6-phosphofructokinase, platelet type) is also named as PFKF,PFK-C and belongs to the phosphofructokinase family.This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK(Francke (1983)).PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. PFKP 抗体
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