DNase I,脱氧核糖核酸酶1抗体

产品编号HZ-7651R
英文名称DNase I
中文名称脱氧核糖核酸酶1抗体
别 名RNASE1; Dornase alfa; Deoxyribonuclease 1; Deoxyribonuclease I; Deoxyribonuclease1; DeoxyribonucleaseI; DNASE 1; DNase I lysosomal; DNASE1; DNaseI; DNL 1; DNL1; DRNI; Human urine deoxyribonuclease I; DNAS1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 细胞凋亡 细胞周期蛋白 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Pig, Horse, Rabbit,
DNase I,脱氧核糖核酸酶1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量29kDa
细胞定位细胞核 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human DNase I
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
DNase I,脱氧核糖核酸酶1抗体PubMedPubMed
产品介绍background:
Deoxyribonuclease I gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.

Subcellular Location:
Secreted. Nucleus envelope.

Tissue Specificity:
Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.

Similarity:
Belongs to the DNase I family.

Gene ID:
1773

Database links:
Entrez Gene: 1773 Human
Omim: 125505 Human
SwissProt: P24855 Human
Unigene: 629638 Human

DNase I,脱氧核糖核酸酶1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.