FOXN1,叉头蛋白N1抗体

产品编号HZ-6970R
英文名称FOXN1
中文名称叉头蛋白N1抗体
别 名FKHL20; Forkhead box N1; Forkhead box protein N1; FOXN 1; FOXN1; FOXN1_HUMAN; RONU; Rowett nude; Transcription factor winged-helix nude; WHN; Winged helix nude; Winged-helix transcription factor nude.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 染色质和核信号 转录调节因子 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
FOXN1,叉头蛋白N1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量69kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human FOXN1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
FOXN1,叉头蛋白N1抗体PubMedPubMed
产品介绍background:
Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq, Jul 2008].

Function:
Transcriptional regulator involved in development.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in thymus.

Similarity:
Contains 1 fork-head DNA-binding domain.

Database links:
UniProtKB/Swiss-Prot: O15353.1

FOXN1,叉头蛋白N1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-FOXN1 Polyclonal Antibody, Unconjugated(HZ-6970R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Protein: rat thymus lysates, 30ug;  Primary: Anti-alpha-FOXN1 (HZ-6970R) at 1:200;  Secondary: HRP conjugated Goat Anti-Rabbit IgG(HZ-0295G-HRP) at 1: 5000;  ECL excitated the fluorescence;  Predicted band size : 69kD Observed band size : 69kD