Factor VII heavy chain,凝血因子7抗体-抗体-抗体-生物在线
Factor VII heavy chain,凝血因子7抗体

Factor VII heavy chain,凝血因子7抗体

商家询价

产品名称: Factor VII heavy chain,凝血因子7抗体

英文名称: Anti-Factor VII heavy chain antibody

产品编号: HZ-4846R

产品价格: null

产品产地: 中国/美国

品牌商标: HZbscience

更新时间: 2023-08-17T10:24:20

使用范围: WB,ELISA,IHC-P,IHC-F,IF

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Factor VII heavy chain,凝血因子7抗体

产品编号HZ-4846R
英文名称Factor VII heavy chain
中文名称凝血因子7抗体
别 名Factor VII heavy chain; coagulation factor VII (serum prothrombin conversion accelerator); Coagulation factor VII; Eptacog alfa; F7; FA7_HUMAN; Factor VII; Factor VII light chain; FVII coagulation protein; OTTHUMP00000018733; OTTHUMP00000018734; Proconvertin; Serum prothrombin conversion accelerator; SPCA.
说 明 书0.1ml 0.2ml
研究领域心血管 细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human,
Factor VII heavy chain,凝血因子7抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量17/28kDa
细胞定位分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Factor VII heavy chain
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Factor VII heavy chain,凝血因子7抗体PubMedPubMed
产品介绍background:
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.

Function:
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.

Subunit:
Heterodimer of a light chain and a heavy chain linked by a disulfide bond.

Subcellular Location:
Secreted.

Tissue Specificity:
Plasma.

Factor VII heavy chain,凝血因子7抗体Post-translational modifications:
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

DISEASE:
Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. A hemorrhagic disease with variable presentation. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or repeated hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Finally, numerous subjects are completely asymptomatic despite very low factor VII levels.

Similarity:
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.

Gene ID:
2155

Database links:
Entrez Gene: 2155 Human
Omim: 613878 Human
SwissProt: P08709 Human
Unigene: 36989 Human

Factor VII heavy chain,凝血因子7抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

凝血因子7是肝脏合成的一种维生素K依赖性的单链糖蛋白,由4 0 6个氨基酸残基组成,分子量约45kD。 凝血因子Ⅶ的半衰期最短(4~6h),血浆含量较低(0.5~2mg/L),故可作为肝病患者蛋白质合成功能减退的早期诊断指标。在慢性肝病患者通过肝活检组织原位杂交的方法检测到凝血因子Ⅶ的表达与肝纤维化的分级呈负相关,可作为预测纤维化程度的指标。凝血因子Ⅶ活性还与预后有着密切的联系,经研究认为:凝血因子Ⅶ活性<34%的肝硬化患者93%在随访10月内死亡,故认为它是肝硬化患者预后好坏的早期预测指标,可更好识别肝移植候选人。肝硬化患者凝血因子Ⅶ活性可明显下降,凝血因子Ⅶ缺乏可导致血小板活性的改变,结合血小板计数减少使出血时间延长,因此对有创诊断与治疗的肝硬化患者,还应该用凝血因子Ⅶ活性进行出血危险度的评估,而不能仅看血小板计数。除诊断之外,重组凝血因子Ⅶ可以有效地纠正肝病患者凝血异常,有利于有创性检查的进行。

产品图片
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-Factor VII Polyclonal Antibody, Unconjugated(HZ-4846R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining