Hexokinase II,己糖激酶2抗体

产品编号HZ-9455R
英文名称Hexokinase II
中文名称己糖激酶2抗体
别 名Hexokinase 2; Hexokinase 2 muscle; Hexokinase type II; Hexokinase-2; HK 2; HK II; HK2; HKII; HxK 2; HxK2; HXK2_HUMAN; Muscle form hexokinase.
说 明 书0.1ml 0.2ml
研究领域肿瘤 心血管 细胞生物 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
Hexokinase II,己糖激酶2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量102kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Hexokinase II/HxK2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Hexokinase II,己糖激酶2抗体PubMedPubMed
产品介绍background:
The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the “set point” for insulin secretion.

Subunit:
Monomer (By similarity).

Subcellular Location:
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.

Tissue Specificity:
Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.

Similarity:
Belongs to the hexokinase family.

Database links:
Entrez Gene: 3099 Human
Omim: 601125 Human
SwissProt: P52789 Human
Unigene: 406266 Human
Unigene: 591588 Human

Hexokinase II,己糖激酶2抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.