Anti-EGFR (N826S) Mouse Monoclonal Antibody-抗体-抗体-生物在线
武汉纽斯特生物技术有限公司
Anti-EGFR (N826S) Mouse Monoclonal Antibody

Anti-EGFR (N826S) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-EGFR (N826S) Mouse Monoclonal Antibody

英文名称： Anti-EGFR (N826S) Mouse Monoclonal Antibody

产品编号： 26116

产品价格： null

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IF、IHC

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
  • 邮编 : 430074
  • 所在区域 : 湖北
  • 电话 : 186****7297 点击查看
  • 传真 : 点击查看
  • 邮箱 : neweast3@126.com
进入展台

 

EGFR (N826S) [26116]
[100 µL]		  

Catalog Number:  26116
Gene Symbol:  EGFR/Her1/Neu
Description:   Anti-EGFR (N826S) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of N826S, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:2000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR (N826S), but not wild type EGFR protein from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of recombinant EGFR(N826S) and wildtype proteins. Purified His-tagged EGFR (N826S) protein (702-987) (Cat. #10221) and corresponding wild-type protein (702-987) (Cat. #10210) were blotted with anti- EGFR (N826S) mouse antibody (Cat. #26116).
Immunofluorescence:  
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (N826S) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (N826S) plasmid (right column), then fixed and stained with anti- EGFR (N826S) monoclonal antibody (Cat. #26116).
Publications:
1.  Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma
    Tumor Biology. October 2014