Anti-IDH2 Rabbit Polyclonal Antibody-抗体-抗体-生物在线
Anti-IDH2 Rabbit Polyclonal Antibody

Anti-IDH2 Rabbit Polyclonal Antibody

商家询价

产品名称： Anti-IDH2 Rabbit Polyclonal Antibody

英文名称： Anti-IDH2 Rabbit Polyclonal Antibody

产品编号： 21278

产品价格： null

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA, WB, IHC

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
  • 邮编 : 430074
  • 所在区域 : 湖北
  • 电话 : 186****7297
  • 传真 : 027-87561022
  • 邮箱 : neweast3@126.com
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IDH2 [21278]
[100 µL]		  

Catalog Number:  21278
Gene Symbol:  IDH2
Description:   Anti-IDH2 Rabbit Polyclonal Antibody
Background:    Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
Immunogen:   Recombinant protein of human IDH2 (full length).
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:1000-1:5000 
IHC: 1:50-1:500
Concentration:    0.1mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-IDH2 antibody recognizes IDH2 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of recombinant IDH2 protein. Purified His-tagged IDH2 protein (full length) (Cat. #10200) was blotted with anti-IDH2 rabbit polyclonal antibody (Cat. # 21278).
Publications:
1.  Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
    Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
2.  Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
    Nature 513, 110–114 (04 September 2014)
3.  Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
    Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015