甲胎蛋白单克隆抗体（包被）
产品名称： 甲胎蛋白单克隆抗体（包被）
英文名称： AFP(A2)
产品编号： hzm-
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
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Mouse Anti-AFP(A2) antibody
| 产品编号 | hzm-1621M |
| 英文名称 | AFP(A2) |
| 中文名称 | 甲胎蛋白单克隆抗体（包被） |
| 别 名 | Alpha Fetoprotein; AFP; alpha 1 fetoprotein; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP. |
| 研究领域 | 肿瘤 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | A2 |
| 交叉反应 | Human, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 65kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Full length plasma AFP protein:full length |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 储 存 液 | 0.01M PBS(pH7.4) with 0.03% Proclin300. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. Subunit: Dimeric and trimeric forms have been found in addition to the monomeric form. Subcellular Location: Secreted. Tissue Specificity: Plasma. Synthesized by the fetal liver and yolk sac. Similarity: Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. SWISS: P02771 Gene ID: 174 Database links: Entrez Gene: 174 Human Omim: 104150 Human SwissProt: P02771 Human Unigene: 518808 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 。 AFP敏感度较低，特异性强，大约60%左右的肝细胞肝癌阳性，在低分化肿瘤中常呈阳性表达。 该抗体与bsm-1622M（检测）（Anti-Alpha Fetoprotein）配对使用；主要用于ELISA试剂盒，胶体金试剂条制备生产都很好。大量订购可来电商议。 |
| 产品图片 |
 Sample: AFP recombinded protein lysate at 100ng;
Primary: Anti-AFP(A4) (bsm-1622M) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-mouse IgG(bs-0296G-HRP) at 1: 5000 dilution; Predicted band size: 65 kD Observed band size: 65 kD  Sample: AFP recombinded protein lysate at 100ng; Primary: Anti-AFP(A4) (bsm-1622M) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-mouse IgG(bs-0296G-HRP) at 1: 5000 dilution; Predicted band size: 65 kD Observed band size: 65 kD
 Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AFP(A2)) Polyclonal Antibody, Unconjugated (bsm-1621M) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
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