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鼠抗人前列腺特异性抗原单克隆抗体(检测)上海沪震实业有限公司

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产品名称: 鼠抗人前列腺特异性抗原单克隆抗体(检测)
英文名称: PSA (PA5)
抗体货号: hzm-1626M
产品规格: 50ul/100ul
级    别: , 分析纯,
产品产地: 中国/上海
品牌商标: HZbscience
价    格: 860-1580元
抗原: IgG
抗原来源: Mouse
抗体来源: Mouse
适用物种: 见说明书
应用范围: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
更新时间: 2020/11/23 11:26:00
详细资料:  实验方法技术资料
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使用范围:仅限科研使用,不能应用于临床
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供应商联系卡

上海沪震实业有限公司
地址:
上海市杨浦区密云路1018号复旦科技园808室
邮编:
200612
电话:
021-60345367
传真:
021-31320307
联系人:
陈先生
所在区域:
上海·中国-上海
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产品详细描述

 Mouse Anti-PSA (PA5) antibody

 

产品编号 hzm-1626M
英文名称 PSA (PA5)
中文名称 鼠抗人前列腺特异性抗原单克隆抗体(检测)
别    名 antigen, prostate-specific; APS; Gamma seminoprotein; Gamma-seminoprotein; hK3; Kallikrein 3; Kallikrein 3, (prostate specific antigen); Kallikrein related peptidase 3; Kallikrein-3; KLK 3; KLK2A1; KLK3; KLK3_HUMAN; P 30 antigen; P-30 antigen; P30 antigen; Prostate Specific Antigen; Prostate-specific antigen; PSA; Semenogelase; Seminin.  
 
  
 
   
研究领域 肿瘤  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 PA5
交叉反应 Human, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 23kDa
细胞定位 分泌型蛋白 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 Full length native protein purified from human:full length 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS(pH7.4) with 0.03% Proclin300
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].

Function:
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Subunit:
Forms heterodimer with SERPINA5.

Subcellular Location:
Secreted.

Tissue Specificity:
Prostate.

Similarity:
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.

SWISS:
P07288

Gene ID:
354

Database links:

Entrez Gene: 354 Human

Omim: 176820 Human

SwissProt: P07288 Human

Unigene: 171995 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

PSA-前列腺特异性抗原为激肽释放酶家族蛋白,为丝氨酸蛋白酶,由前列腺上皮产生,精液中的浓度很高,可使精液凝块水解、液化,功能与男性生育力有关.
此抗体可用于试剂盒检测用抗体。
产品图片
Sample: DU145(Human) Lysate at 30 ug
Primary: Anti-PSA (PA5) (bsm-1626M-HRP) at 1/1000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
Tissue/cell: human prostate carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-PSA(PA5) Polyclonal Antibody, Unconjugated(bsm-1626M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining

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