Rabbit Anti-phospho-Cyclin D3 (Thr283)/FITC Conjugated antibody
|英文名称||Anti-phospho-Cyclin D3 (Thr283)/FITC|
|别 名||Cyclin D3 (phospho T283); Cyclin D3 (phospho Thr283); p-Cyclin D3 (T283); p-Cyclin D3 (Thr283); p-Cyclin D3 (phospho T283); CCND 3; Ccnd3; CCND3_HUMAN; CyclinD3; D3 type cyclin; G1 S specific cyclin D3; G1/S specific cyclin D3; G1/S-specific cyclin-D3.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||肿瘤 细胞生物 染色质和核信号 细胞周期蛋白 表观遗传学|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||33kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human Cyclin D3 around the phosphorylation site of Thr283|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008].
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K. Component of the ternary complex cyclin D/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity. Can form similar complexes with either CDKN1A or CDKN2A.
Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.
Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.
Belongs to the cyclin family. Cyclin D subfamily.
Contains 1 cyclin N-terminal domain.
Entrez Gene: 540547 Cow
Entrez Gene: 896 Human
Entrez Gene: 12445 Mouse
Entrez Gene: 25193 Rat
Omim: 123834 Human
SwissProt: Q3MHH5 Cow
SwissProt: P30281 Human
SwissProt: P30282 Mouse
SwissProt: P48961 Rat
Unigene: 534307 Human
Unigene: 27291 Mouse
Unigene: 472101 Mouse
Unigene: 3483 Rat
Unigene: 54319 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
该基因编码的蛋白属于高度保守的cyclin家族，其成员特征是细胞周期中蛋白丰度的显著周期性。细胞周期蛋白是CDK激酶的调节因子。不同的周期蛋白表现出不同的表达和降解模式，这有助于每个有丝分裂事件的时间协调。该细胞周期蛋白与CDK4或CDK6形成复合物，作为调节亚基发挥作用，其活性是细胞周期G1/S转变所必需的。该蛋白已被证明与肿瘤抑制蛋白Rb的***酸化相互作用和参与。据报道，与该细胞周期素相关的CDK4活性对于细胞周期在紫外线照射后通过G2期进入有丝分裂是必需的。已经发现了几种编码不同亚型的转录本变体。[ RefSeq，OCT 2008 ]提供。