|
Anti-MAP Kinase 1/2 (Erk1/2) Rabbit Polyclonal Antibody
Catalog No: KC-5E01 Size: 100 µl (10 Western mini-blots) |
|
Applications |
Species Cross-Reactivity |
Molecular Wt. |
Source |
|
W, IHC, F |
H, M, R, Hm, Z, Ch, starfish |
42, 44 kDa |
Rabbit |
Background:
|
Western blot analysis of extracts using p44/42 MAP Kinase Antibody. |
Specificity/Sensitivity:
Erk 1/2 MAP Kinase Antibody detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. The antibody does not recognize either JNK/SAPK or p38 MAP kinase.
Source/Purification:
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH coupled) corresponding to the C-terminal 35 amino acids of rat p44 MAP kinase. Antibodies are purified by protein A and peptide affinity chromatography.
Storage and Stability:
Each vial contains 100ul affinity purified rabbit anti-MAP Kinase 1/2 (Erk1/2) antibody in a special stabilizer. Real time studies that this special stabilizer helped retain most of antibody activity for 24 months while store at 4℃.
Recommended Antibody Dilutions:
|
Western blotting |
1:1000 |
|
Immunohistochemistry (Paraffin) |
1:100 |
|
Flow cytometry |
1:25 |