HITRAP BLUE HP-色谱柱 -耗材-生物在线
HITRAP BLUE HP

HITRAP BLUE HP

商家询价

产品名称： HITRAP BLUE HP

英文名称： HITRAP BLUE HP

产品编号： 17-0412-01

产品价格： 0

产品产地： USA

品牌商标： 美国GE

更新时间： null

使用范围： null

上海基星生物科技有限公司
  • 联系人 :
  • 地址 : 上海市宝山区友谊路1506号东智广场1109室
  • 邮编 : 201900
  • 所在区域 : 上海
  • 电话 : 138****5983
  • 传真 : 021-50801884
  • 邮箱 : 342786402@qq.com
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17-0412-01 HITRAP BLUE HP, 5 X 1 ML
17-0413-01 HITRAP BLUE HP, 1 X 5 ML
17-0716-01 HITRAP NHS-ACTIV HP, 5 X 1ML
17-0716-30 HiTrap NHS-activated HP, 180 x
17-0717-01 HITRAP NHS-ACTIV HP, 1 X 5 ML
17-0920-03 HITRAP IMAC HP, 5 X 1 ML
17-0920-05 HITRAP IMAC HP, 5 X 5 ML
17-0921-02 HITRAP IMAC FF, 5 X 1ML
17-0921-04 HITRAP IMAC FF, 5 X 5 ML
17-0978-13 HITRAP BUTYL-S FF, 5 X 1ML
17-0978-14 HITRAP BUTYL-S FF, 5 X 5ML
17-1128-01 MABTRAP KIT
17-1151-01 HITRAP SP HP, 5 X 1 ML
17-1152-01 HITRAP SP HP, 5X5 ML
17-1153-01 HITRAP Q HP, 5 X 1 ML
17-1154-01 HITRAP Q HP 5X5 ML
17-1351-01 HITRAP PHENYL HP, 5 X 1ML
17-1353-01 HITRAP PHENYL FF (LS), 5X1ML
17-1355-01 HITRAP PHENYL FF (HS),5 X 1ML
17-1357-01 HITRAP BUTYL FF, 5 X 1 ML
17-1359-01 HITRAP OCTYL FF, 5 X 1 ML
17-1408-01 HITRAP DESALTING, 5X5ML
17-5053-01 HITRAP Q FF, 5 X 1 ML
17-5054-01 HITRAP SP FF, 5 X 1 ML
17-5055-01 HITRAP DEAE FF 5 X 1 ML
17-5056-01 HITRAP CM FF, 5 X 1 ML
17-5079-01 HITRAP RPROTEIN A FF, 5X1ML
17-5079-02 HITRAP RPROTEIN A FF, 2 X 1 ML
17-5080-01 HITRAP RPROTEIN A FF, 1X5ML
17-5080-02 HITRAP RPROTEIN A FF, 5 X 5 ML
17-5110-01 HITRAP IGM PURIFICATION 5X1ML
17-5111-01 HITRAP IGY PURIFICATION 1X5 ML
17-5112-01 HITRAP STREPTAVIDIN HP5X1 ML
17-5130-01 GSTRAP FF, 5X1ML
17-5130-02 GSTRAP FF, 2X1ML
17-5130-05 GSTRAP FF, 100 X 1 ML
17-5131-01 GSTRAP FF, 1X5ML
17-5131-02 GSTRAP FF 5 X 5 ML
17-5131-05 GSTRAP FF, 100 X 5 ML
17-5143-01 HITRAP BENZAMIDINE FF 5 X 1ML
17-5143-02 HITRAP BENZAMIDINE FF 2 X 1 ML
17-5144-01 HITRAP BENZAMIDINE FF 1 X 5 ML
17-5154-01 HITRAP DEAE FF 5 X 5 ML
17-5155-01 HITRAP CM FF, 5 X 5 ML
17-5156-01 HITRAP Q FF, 5 X 5 ML
17-5157-01 HITRAP SP FF, 5 X 5 ML
17-5158-01 HITRAP Q XL, 5 X 1 ML
17-5159-01 HITRAP Q XL, 5 X 5 ML
17-5160-01 HITRAP SP XL, 5 X 1 ML
17-5161-01 HITRAP SP XL, 5 X 5 ML
17-5162-01 HITRAP ANX FF (HIGHSUB)5 X 1ML
17-5163-01 HITRAP ANX FF(HIGHSUB) 5 X 5ML
17-5193-01 HITRAP PHENYL FF (HS), 5 X 5ML
17-5194-01 HITRAP PHENYL FF (LS), 5X 5ML
17-5195-01 HITRAP PHENYL HP, 5 X 5 ML
17-5196-01 HITRAP OCTYL FF, 5 X 5 ML
17-5197-01 HITRAP BUTYL FF, 5 X 5 ML
17-5247-01 HISTRAP HP 5 X 1 ML
17-5247-05 HISTRAP HP 100 X 1 ML
17-5248-01 HISTRAP HP 1 X 5 ML
17-5248-02 HISTRAP HP 5 X 5 ML
17-5248-05 HISTRAP HP 100 X 5 ML
17-5255-01 HISTRAP FF 5 X 5 ML
17-5255-02 HISTRAP FF 100 X 5 ML
17-5281-01 GSTRAP HP 5 X 1 ML
17-5281-05 GSTRAP HP 100 X 1 ML
17-5282-01 GSTRAP HP 1 X 5 ML
17-5282-02 GSTRAP HP 5 X 5 ML
17-5282-05 GSTRAP HP, 100 X 5 ML
17-5286-01 HISTRAP FF CRUDE 5X5 ML
17-5286-02 HISTRAP FF CRUDE 100X5 ML
17-5319-01 HISTRAP FF 5 X 1 ML
17-5319-02 HISTRAP FF 100 X 1 ML
17-5441-22 HITRAP CAPTO S, 5X1 ML
17-5441-23 HITRAP CAPTO S, 5X5 ML
17-6002-33 HITRAP IEX SELECTION KIT
18-1142-75 REC PROTEIN PURIF HANDBOOK
28-4010-81 HITRAP_HIPREP 1_16 CONNEC AKTA
28-4014-77 HISTRAP FF CRUDE KIT
28-4017-45 GSTRAP 4B, 5 X 1 ML
28-4017-46 GSTRAP 4B, 100 X 1 ML
28-4017-47 GSTRAP 4B, 1 X 5 ML
28-4017-48 GSTRAP 4B, 5 X 5 ML
28-4017-49 GSTRAP 4B, 100 X 5 ML
28-4058-44 HiTrap Capto Adhere 5x1ml
28-4058-46 5x5 ml Hitrap Capto adhere
28-4082-53 HITRAP MABSELECT, 5 X 1 ML
28-4082-55 HITRAP MABSELECT, 1 X 5 ML
28-4082-56 HITRAP MABSELECT, 5 X 5 ML
28-4082-58 HITRAP MABSELECT XTRA, 5X1ML
28-4082-60 HITRAP MABSELECT XTRA, 1X5ML
28-4082-61 HITRAP MABSELECT XTRA, 5X5ML
28-4110-01 HiTrap Butyl HP, 5x1ml
28-4110-05 HiTrap Butyl HP, 5 x 5ml
28-4110-07 HiTrap HIC Selection Kit, 7x1

HiTrap™ Blue HP Columns

  • Prepacked with Blue Sepharose™ High Performance for purifying albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons, and related proteins.
  • Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked agarose medium and is stable over a pH range of 4-12 for use and 3-13 for cleaning.

HiTrap™ Blue HP Columns

Technical Information


Click here to view this image in high resolution
Blue Sepharose™ High Performance is a group specific adsorbent with affinity for a wide variety of enzymes. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the matrix via the triazine part of the dye molecule. Some proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors, while others, such as albumin and interferon, bind in a less specific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand.

 

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Lane 1: Low Molecular Weight (LMW) calibration kit, reduced
Lane 2: Human serum, buffer exchanged, non-reduced, diluted 1:50
Lane 3: Pool I, non-reduced, diluted 1:10
Lane 4: Pool II, non-reduced, diluted 1:10.
 

 

The use of HiTrap™ Blue is illustrated by the removal of albumin from human serum. The contents of the different fractions were analysed by SDS-PAGE using PhastSystem™ and silver staining. The separation took approximately 40 min.
Scale-up is easily done by coupling 2-4 HiTrap™ Blue Columns in series.
 

 

Click here to view this image in high resolution
Removal of albumin from human serum using HiTrap™ Blue HP, 5 ml.