HiScreen MabSelectXtra
产品名称： HiScreen MabSelectXtra
英文名称： HiScreen MabSelectXtra
产品编号： 28-9269-76
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
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- 地址 : 上海市宝山区友谊路1506号东智广场1109室
- 邮编 : 201900
- 所在区域 : 上海
- 电话 : 138****5983
- 传真 : 021-50801884
- 邮箱 : 342786402@qq.com
| 28-9269-76 | HiScreen MabSelectXtra |
| 28-9269-77 | HiScreen MabSelectSuRe |
| 28-9269-78 | HiScreen Capto Q |
| 28-9269-79 | HiScreen Capto S |
| 28-9269-80 | HiScreen Capto MMC |
| 28-9269-81 | HiScreen Capto Adhere |
| 28-9269-82 | HiScreen Capto DEAE |
| 28-9269-84 | HiScreen Butyl FF |
| 28-9269-85 | HiScreen Butyl-S FF |
| 28-9269-86 | HiScreen Octyl FF |
| 28-9269-88 | HiScreen Phenyl FF, high sub |
| 28-9269-89 | HiScreen Phenyl FF, low sub |
HiScreen™ MabSelect™ Columns
Technical Information
HiScreen MabSelect columns are part of the process development platform available from GE Healthcare. Four prepacked HiScreen columns with MabSelect SuRe™ LX, MabSelect SuRe, MabSelect, and MabSelect Xtra™ are available as ideal columns for capture of monoclonal antibodies (MAbs).
Due to the small column volume (4.7 ml) and 10 cm bed height, these columns are excellent tools for method optimization, parameter screening, robustness testing, and convenient scaling up. Process flow velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm.
Chromatography media prepacked in different HiScreen columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.
MabSelect is designed for fast purification of MAbs from large sample volumes. It is compatible with high flow rates and high pressure when scaling up.
MabSelect Xtra is designed for higher binding capacity, allowing binding from samples with high expression levels of the MAb.
MabSelect SuRe is designed with an alkali-tolerant, protein A-derived ligand, allowing the use of 0.1 to 0.5 M sodium hydroxide for cleaning-in-place (CIP). It has a high binding capacity and is a good choice for feed streams with typical titers of ~1 to 3 g/l.
MabSelect SuRe LX has an exceptional high dynamic binding capacity for high-titer cultures of antibodies (> 3 g/l) at longer residence times. MabSelect SuRe LX is designed with the same alkali-tolerant, protein A-derived ligand as in MabSelect SuRe, which makes the medium stable in sodium hydroxide concentrations as high as 0.5 M.
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HiScreen columns prepacked with MabSelect media for convenient and robust process development of monoclonal antibodies (MAbs).
 to XK 16/40 packed with MabSelect SuRe (orange). Overlay of UV absorbance curves for eluted peaks.'))
Scaling up 4.6 times from HiScreen MabSelect SuRe (blue) to XK 16/40 packed with MabSelect SuRe (orange). Overlay of UV absorbance curves for eluted peaks.
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| TECHNICAL SPECIFICATIONS | |
| Media | MabSelect SuRe LX MabSelect SuRe MabSelect MabSelect Xtra |
| Column volume | 4.7 ml |
| Column dimension | 0.77 × 10 cm |
| Recommended flow rates | 0.6-1.2 ml/min (75-150 cm/h); MabSelect SuRe LX 1-1.2 ml/min(120-150 cm/h); MabSelect SuRe 1-2 ml/min (120-250 cm/h); MabSelect 0.4-1.2 ml/min (50-150 cm/h); MabSelect Xtra |
| Maximum flow rates† | 3.9 ml/min (500 cm/h); MabSelect SuRe LX, MabSelect SuRe, and MabSelect 2.3 ml/min (300 cm/h); MabSelect Xtra |
| Column hardware pressure limit | 8 bar (0.8 MPa, 117 psi) |
| Maximum pressure over the packed bed during operation, Dp† | |
| MabSelect, MabSelect Xtra, MabSelect SuRe MabSelect SuRe LX |
3 bar (0.3 MPa, 44 psi) 3 bar (0.3 MPa, 44 psi) |
| †Water at room temperature | |
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