HisPrep FF 16/10-色谱柱 -耗材-生物在线
HisPrep FF 16/10

HisPrep FF 16/10

商家询价

产品名称： HisPrep FF 16/10

英文名称： HisPrep FF 16/10

产品编号： 28-9365-51

产品价格： 0

产品产地： 美国

品牌商标： GE

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使用范围： null

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28-9365-51 HisPrep FF 16/10 1 ×20 ml

 

Use 20-40 mM imidazole in sample and binding buffers for highest purity.
* Pack size available by special order.

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HisPrep FF 16/10 columns are prepacked with Ni Sepharose 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow consists of 90-μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

 

HisPrep™ FF 16/10 column for convenient scale-up purification of histidine-tagged proteins.

 

Customer feedback on Ni Sepharose 6 Fast Flow.

 

* For licensing information, see Legal Info.

 

HiPrep IMAC FF 16/10, see IMAC Sepharose 6 Fast Flow Media/HiTrap IMAC FF Columns/HiPrep IMAC FF 16/10 Column/HiScreen IMAC FF Column, is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

For the complete range of products for purification of histidine-tagged proteins, see Introduction to Tagged Protein Purification.

 


 

HisPrep™ FF 16/10 Column - Easy Scale-up Purification

Technical Information


TECHNICAL SPECIFICATIONS
Medium  Ni Sepharose 6 Fast Flow 
Column volume  20 ml 
Dynamic binding capacity*  Approx. 40 mg histidine-tagged protein/ml medium 
Column dimensions  1.6 × 10 cm 
Recommended flow rate†  2-10 ml/min (60-300 cm/h) 
Max. flow rate†  10 ml/min (300 cm/h) 
Max. pressure over the packed bed during operation, Dp  0.15 MPa, 1.5 bar 
Column hardware pressure limit  0.5 MPa, 5 bar 
Compatibility  Stable in all commonly used buffers, reducing agents, denaturants, and detergents, see Introduction to Tagged Protein Purification for more information. 
Storage  20% ethanol 
Storage temperature  4°C to 30°C 
* Binding capacity is protein-to-protein dependent
† H2O at room temperature

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