HITRAP Q HP-色谱柱 -耗材-生物在线
HITRAP Q HP

HITRAP Q HP

商家询价

产品名称: HITRAP Q HP

英文名称: HITRAP Q HP

产品编号: 17-1153-01

产品价格: 0

产品产地: 美国

品牌商标: GE

更新时间: null

使用范围: null

上海基星生物科技有限公司
  • 联系人 :
  • 地址 : 上海市宝山区友谊路1506号东智广场1109室
  • 邮编 : 201900
  • 所在区域 : 上海
  • 电话 : 138****5983
  • 传真 : 021-50801884
  • 邮箱 : 342786402@qq.com

17-1153-01 HITRAP Q HP, 5 X 1 ML
17-1154-01 HITRAP Q HP 5X5 ML
17-1351-01 HITRAP PHENYL HP, 5 X 1ML
17-1353-01 HITRAP PHENYL FF (LS), 5X1ML
17-1355-01 HITRAP PHENYL FF (HS),5 X 1ML
17-1357-01 HITRAP BUTYL FF, 5 X 1 ML
17-1359-01 HITRAP OCTYL FF, 5 X 1 ML
17-1408-01 HITRAP DESALTING, 5X5ML
17-5053-01 HITRAP Q FF, 5 X 1 ML
17-5054-01 HITRAP SP FF, 5 X 1 ML
17-5055-01 HITRAP DEAE FF 5 X 1 ML
17-5056-01 HITRAP CM FF, 5 X 1 ML
17-5079-01 HITRAP RPROTEIN A FF, 5X1ML
17-5079-02 HITRAP RPROTEIN A FF, 2 X 1 ML
17-5080-01 HITRAP RPROTEIN A FF, 1X5ML
17-5080-02 HITRAP RPROTEIN A FF, 5 X 5 ML
17-5110-01 HITRAP IGM PURIFICATION 5X1ML
17-5111-01 HITRAP IGY PURIFICATION 1X5 ML
17-5112-01 HITRAP STREPTAVIDIN HP5X1 ML
17-5130-01 GSTRAP FF, 5X1ML
17-5130-02 GSTRAP FF, 2X1ML
17-5130-05 GSTRAP FF, 100 X 1 ML

HiTrap™ IEX HP Columns

  • Prepacked with Q Sepharose™ High Performance or SP Sepharose™ High Performance for high resolution purifications on 34-μm beads.
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
  • Designed for use with a syringe, peristaltic pump and chromatography system such as ÄKTA™design.

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HiTrap™ IEX HP Columns

Technical Information


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HiTrap™ SP HP and HiTrap™ Q HP ion exchange columns are fast and convenient to use.

 

The ion exchange media packed in HiTrap™ ion exchange columns are based on Sepharose™ High Performance. The small particle size (34μm) allows fast adsorption and desorption even at high sample loadings and flow rates. SP Sepharose™ High Performance is a strong cation exchange medium and Q Sepharose™ High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.

 

TECHNICAL SPECIFICATIONS
HiTrap™ SP HP and HiTrap™ Q HP
Media  SP Sepharose™ High Performance
Q Sepharose™ High Performance 
Column volume  1 ml or 5 ml 
Max. flow rate   
1-ml column  4 ml/min 
5-ml column  20 ml/min 
Recommended flow rate 
1-ml column  1 ml/min 
5-ml column  5 ml/min 
Max. back pressure  3 bar (43 psi, 0.3 MPa) 
Storage  20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP) 
Storage temperature  4°C to 30°C 
   

 

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Separation of human serum proteins on a 1 ml HiTrap™ Q column using a one-step gradient. The separation conditions were optimised for the binding of serum components other than the IgG fraction, which passed through. The result of a subsequent SDS-electrophoresis analysis is shown below.
 

 

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SDS -PAGE electrophoresis with PhastSystem™ and PhastGel™ Gradient 8-25, Coomassie™ stained. The separation conditions were optimised for the binding of serum components other than the IgG fraction, which passed through. The result of a subsequent SDS-electrophoresis analysis is shown below.
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