HITRAP MABSELECT-色谱柱 -耗材-生物在线
HITRAP MABSELECT

HITRAP MABSELECT

商家询价

产品名称: HITRAP MABSELECT

英文名称: HITRAP MABSELECT

产品编号: 28-4082-53

产品价格: 0

产品产地: 美国

品牌商标: GE

更新时间: null

使用范围: null

上海基星生物科技有限公司
  • 联系人 :
  • 地址 : 上海市宝山区友谊路1506号东智广场1109室
  • 邮编 : 201900
  • 所在区域 : 上海
  • 电话 : 138****5983
  • 传真 : 021-50801884
  • 邮箱 : 342786402@qq.com

28-4082-53 HITRAP MABSELECT, 5 X 1 ML
28-4082-55 HITRAP MABSELECT, 1 X 5 ML
28-4082-56 HITRAP MABSELECT, 5 X 5 ML
28-4082-58 HITRAP MABSELECT XTRA, 5X1ML
28-4082-60 HITRAP MABSELECT XTRA, 1X5ML
28-4082-61 HITRAP MABSELECT XTRA, 5X5ML
28-4110-01 HiTrap Butyl HP, 5x1ml
28-4110-05 HiTrap Butyl HP, 5 x 5ml
28-4110-07 HiTrap HIC Selection Kit, 7x1
28-9075-46 StrepTrap HP 5 x 1mL
28-9075-47 StrepTrap HP 1 x 5mL
28-9075-48 StrepTrap HP 5 x 5mL
28-9165-37 HiTrap Capto DEAE, 5X1ML
28-9165-40 HiTrap Capto DEAE, 5X5ML
28-9187-78 MBPTrap 5 x 1mL
28-9187-79 MBPTrap 1 x 5 mL
28-9187-80 MBPTrap 5 x 5mL
28-9343-88 HiTrap Capto IEX Selection Kit
28-9520-85 HiTrap Con A 4B 5 x 1 ml
28-9520-96 HiTrap Con A 4B 5 x 5 ml

MabSelect™ Media and HiTrap™ MabSelect Prepacked Columns

  • MabSelect media allow capture of monoclonal antibodies (Mabs) from large sample volumes.
  • Prepacked HiTrap columns for screening of purification conditions and small-scale purification of Mabs.
  • Media designed to process more than 10 000 l feed from high-expression fermentations in a working day at process scale.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

MabSelect™ Media and HiTrap™ MabSelect Prepacked Columns

Technical Information


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MabSelect Media and HiTrap MabSelect prepacked columns for purification of monoclonal antibodies with optimized binding capacities at high flow rates

 

MabSelect™ SuRe LX is optimized for high dynamic binding capacity for high titer cultures of antibodies. This affinity chromatography medium is designed with the same alkali-tolerant, protein A-derived ligand as in MabSelect SuRe, making it stable in as high concentration as 0.5 M NaOH.

 

MabSelect™ is a BioProcess™ affinity medium for capturing Mabs from large volumes of feed by packed bed chromatography. The recombinant protein A ligand of MabSelect™ is specially engineered to favor an oriented coupling that gives an affinity medium with enhanced binding capacity for IgG. The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification. In addition, the high capacity, low ligand leakage, and the novel base matrix make MabSelect™ ideal for purification of Mabs at process scale. MabSelect™ is available prepacked in 1-ml and 5-ml HiTrap™ MabSelect™ columns for fast purification of Mabs from large sample volumes. The prepacked MabSelect™ medium is compatible with the high flow rates and high pressure required when scaling up.

 

MabSelect™ SuRe has been developed from the same highly cross-linked agarose matrix used for MabSelect™, which enables high flow rates at low backpressure. In contrast to the recombinant protein A ligand of MabSelect™, however, the alkali-tolerant recombinant protein A ligand of MabSelect™ SuRe is resistant to harsh cleaning agents (NaOH), which results in significant cost savings. The high alkaline tolerance of the medium also provides the possibility to extend the number of cycles in regular production.

 

HiTrap™ MabSelect SuRe™ columns are prepacked with MabSelect SuRe™. The high-flow matrix of MabSelect SuRe™ makes HiTrap™ MabSelect SuRe™ well suited for the purification of MAbs when scaling up. HiTrap™ MabSelect SuRe™ columns also allow simulation of cleaning-in-place in process development on account of the alkali-tolerant recombinant Protein A ligand of the prepacked medium.

 

MabSelect Xtra™ has been developed to meet the demands of ever-increasing levels of expression in monoclonal antibody feedstocks. MabSelect Xtra™ uses the same recombinant protein A ligand as MabSelect™, but has a smaller particle size and greater porosity, which ensures increased dynamic binding capacity. The medium provides a lower overall production cost due to the possibility of processing concentrated feedstocks in fewer batches. Process development, screening of purification conditions, and small-scale purification of Mabs can be performed using 1-ml and 5-ml HiTrap™ MabSelect Xtra™ columns.

 

TECHNICAL SPECIFICATIONS
Ligand  Recombinant protein A (MabSelect, MabSelect Xtra), alkali-tolerant recombinant protein A (MabSelect SuRe) 
Ligand coupling method  Epoxy activation 
Matrix  Highly cross-linked agarose 
Average particle size d50V* 
MabSelect  85 μm 
MabSelect SuRe  85 μm 
MabSelect SuRe LX  85 μm 
MabSelect Xtra  75 μm 
Dynamic binding capacity 
MabSelect  At least 30 mg human IgG/ml at 2.4 min residence time 
MabSelect SuRe  At least 30 mg human IgG/ml at 2.4 min residence time 
MabSelect SuRe LX  Approx. 60 mg human IgG/ml medium at 6 min residence time 
MabSelect Xtra  At least 40 mg human IgG/ml at 2.4 min residence time 
Recommended flow velocity 
MabSelect  500 cm/h 
MabSelect SuRe  500 cm/h 
MabSelect SuRe LX  500 cm/h 
MabSelect Xtra  300 cm/h 
pH stability§ 
MabSelect  3-10 (long term), 2-12 (cleaning-in-place) 
MabSelect SuRe  3-12 (long term), 2-14 (cleaning-in-place) 
MabSelect SuRe LX  3-12 (long term), 2-14 (cleaning-in-place) 
MabSelect Xtra  3-10 (long term), 2-12 (cleaning-in-place) 
Storage  20% ethanol 
Storage temperature  2°C to 8°C 
HiTrap column specifications   
Column volume  1 ml and 5 ml 
Column dimensions  0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml)  
Column hardware pressure limit  5 bar (0.5 MPa, 73 psi)  
Recommended fluid rate  1 ml/min (1 ml); 5 ml/min (5 ml)  
* d50V is the median particle size of the cumulative volume distribution.
Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
Determined in BPG 300 column, bed height 20 cm, operating pressure less than 2 bar.
§ A pH below 3 is sometimes required to elute strongly bound IgG species. Note, however, that protein ligands may hydrolyze at very low pH.
Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of
100 cm/h in a column with a bed height of 10 cm.

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