SUPEROSE 6 PC 3.2/30-色谱柱 -耗材-生物在线
SUPEROSE 6 PC 3.2/30

SUPEROSE 6 PC 3.2/30

商家询价

产品名称: SUPEROSE 6 PC 3.2/30

英文名称: SUPEROSE 6 PC 3.2/30

产品编号: 17-0673-01

产品价格: 0

产品产地: 美国

品牌商标: GE

更新时间: null

使用范围: null

上海基星生物科技有限公司
  • 联系人 :
  • 地址 : 上海市宝山区友谊路1506号东智广场1109室
  • 邮编 : 201900
  • 所在区域 : 上海
  • 电话 : 138****5983
  • 传真 : 021-50801884
  • 邮箱 : 342786402@qq.com

17-0671-01 MONO Q PC 1.6/5
17-0672-01 MONO S PC 1.6/5
17-0673-01 SUPEROSE 6 PC 3.2/30
17-0674-01 SUPEROSE 12 PC 3.2/30
17-0686-01 MINI Q PC 3.2/3
17-0687-01 MINI S PC 3.2/3
17-0771-01 SUPERDEX 75 PC 3.2/30
17-1089-01 SUPERDEX 200 PC 3.2/30
17-1455-01 PRECISION COLUMN HOLDER
17-1458-01 SUPERDEX PEPTIDE PC 3.2/30
17-1550-01 COLUMN HOLDER, 10 CM
18-1015-28 PROTECTIVE CAPS (2/PK)
17-5057-01 MICRORPC C2-C18 ST 4,6-100
17-5068-01 SOURCE 15RPC ST 4.6/100
17-5116-01 SOURCE 5RPC ST 4,6-150
17-5172-01 SUPEROSE 6 10/300 GL
17-5173-01 SUPEROSE 12 10/300 GL
17-5174-01 SUPERDEX 75 10/300 GL
17-5175-01 SUPERDEX 200 10/300 GL
17-5176-01 SUPERDEX PEPTIDE 10/300 GL
28-9065-61 Superdex 200 5_150 GL
28-9205-04 Superdex 75 5_150 GL
17-0506-01 PP COLUMN MONO Q HR 16/10
17-0507-01 PP COLUMN MONO S HR 16/10

Superose™ Columns and Lab Packs

  • Achieve high-resolution separations across exceptionally broad molecular-weight ranges.
  • Easy, efficient scale-up from prepacked Superose™ 10/300 GL columns to Superose™ prep grade lab packs.
  • Low ionic strength eluents (< 0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose™ for some lipids, peptides, and small aromatic compounds.
  • Tricorn™ high-performance column (10/300 GL) prepacked with Superose™ allows even distribution of eluent across the column bed to enable high-resolution separations.
  • Precision columns (PC) for micropurification and analysis¾10-fold smaller column volume than Tricorn™ columns

Superose™ Columns and Lab Packs

Technical Information


Superose™ 6 is designed for high-resolution chromatography. Superose™ 6 prep grade permits easy, efficient scale-up to preparative separations. Superose™ 12 is for high resolution purification of proteins from 1 × 103 to 3 × 105 molecular weight, while Superose™ 12 prep grade is useful for preparative purification of these higher molecular weight proteins.

 

TECHNICAL SPECIFICATIONS
Superose™ 10/300 GL Columns (Tricorn™)*
Bed volume  24 ml 
Sample loading capacity  5-10 mg, 240 μl 
Recommended flow rate   
Superose™ 6  0.3-0.5 ml/min 
Superose™ 12  0.5-1.0 ml/min 
Max. pressure   
Superose™ 6  15 bar (217 psi, 1.5 MPa) 
Superose™ 12  30 bar (435 psi, 3 MPa) 
Particle size   
Superose™ 6  13 ± 2 μm 
Superose™ 12  10 ± 2 μm 
Storage  20% ethanol 
Storage temperature  4°C to 30°C 
Chemical stability  Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
1% SDS, organic solvents,
0.5 M NaOH (for cleaning-in-place) 
pH stability  3-12 (working and long term) 
  1-14 (short term) 
Theoretical plates   
Superose™ 6  > 30 000 m-1 
Superose™ 12  > 40 000 m-1 
Superose PC 3.2/30
Bed volume  2.4 ml  
Max. loading capacity  200 μl 
Max. flow rate   
Superose 6  0.1 ml/min 
Superose 12  0.1 ml/min 
Max. pressure   
Superose 6  12 Bar (1.2MPa / 175 psi) 
Superose 12  24 Bar (2.4MPa / 350 psi) 
Particle size   
Superose 6  13 ± 2 μm 
Superose 12  10 ± 2 μm 
Chemical stability  Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place) 
pH stability  3-12 (working and long term) 1-14 (short term) 
Theoretical plates   
Superose 6  > 30 000 m-1 
Superose 12  > 40 000 m-1 
* Columns are not suitable for use with ÄKTAprime™ plus system.

 

 

TECHNICAL SPECIFICATIONS
Superose™ prep grade
Exclusion limit (Mr  
Superose™ 6 prep grade  4 × 107 protein 
Superose™ 12 prep grade  2 × 106 protein 
Separation range (Mr  
Superose™ 6 prep grade  5 × 103-5 × 106 protein 
Superose™ 12 prep grade  1 × 103-3 × 105 protein 
Matrix  Highly cross-linked agarose 
Particle size  30 ± 10 μm 
Recommended linear flow rate   
Superose™ 6 prep grade  up to 40 cm/h 
Superose™ 12 prep grade  up to 40 cm/h 
Max. pressure   
Superose™ 6 prep grade:  4 bar (58 psi, 0.4 MPa) 
  Superose™ 12 prep grade:  7 bar (101 psi, 0.7 MPa) 
Storage  20% ethanol 
Storage temperature  4°C to 30°C 
* Columns are not suitable for use with ÄKTAprime™ plus system.

 

 

Click here to view this image in high resolution
Separation of DNA fragments on Superose™ 6 HR 10/30 column (now available as Superose 6 10/300 GL column). Numbers above peaks correspond to the number of base pairs. Reproduced by kind permission of the authors and publisher.

 

References

 

  1. Andersson, T et al. Agarose-based media for high-resolution gel filtration of  biopolymers. J. Chromatogr. 326, 33 (1985).
  2. Ellegren, H. and Låås, T. Size-exclusion chromatography of DNA restriction fragments.  J. Chromatogr. 467, 217 (1989).
  3. Gao, Y. et al. A cytoplasmic chaperonin that catalyzes beta-actin folding. Cell 69, 1043 (1992).
  4. Lin, M. et al. Pyruvate kinase isoenzymes from Green Alga, Selenastrum minutum. Arch. Biochem. Biophys. 269, 219 (1989).
  5. Hei, Y. et al. Purification and characterisation of a novel ribosomal S6 kinase from skeletal muscle of insulin-treated rats. J. Biol. Chem. 269, 7816 (1994).

详情请查询官网:http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165423