Granzyme B人酶联免疫斑点法(ELISPOT)试剂盒-常用耗材-耗材-生物在线
Granzyme B人酶联免疫斑点法(ELISPOT)试剂盒

Granzyme B人酶联免疫斑点法(ELISPOT)试剂盒

商家询价

产品名称: Granzyme B人酶联免疫斑点法(ELISPOT)试剂盒

英文名称: C11 CGL1 CSPB CTLA1 Fragmentin 2 Granzyme 2 SECT

产品编号: ab46617

产品价格: 0

产品产地: 英国

品牌商标: abcam

更新时间: null

使用范围: null

深圳市宇德立生物科技有限公司
  • 联系人 :
  • 地址 : 深圳市宝安区西乡宝民二路贤基大厦4E
  • 邮编 :
  • 所在区域 : 广东
  • 电话 : 133****4454
  • 传真 : 0755-26457513
  • 邮箱 : 1484332550@qq.com

 

  • 检测方法Colorimetric
  • 测试
    5 x 96 well plate
  • 样品类型
    Cell culture supernatant, Cell culture extracts
  • 检测类型Sandwich (qualitative)
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human

产品概述

This ELISPOT assay is designed to enumerate Granzyme B producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing Granzyme B production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of Granzuyme B producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.

 

The Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

 

Principle of Method

 

After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

组件 5 x 96 tests 10 x 96 tests

96 PVDF-bottomed-well plates. 5 units 10 units
Bovine Serum albumin 1 x 1g 2 x 1g
Dry skimmed milk 1 x 1g 2 x 1g
Granzyme B Biotinylated detection antibody 1 vial 2 vials
Granzyme B Capture Antibody 1 x 500µl 2 x 500µl
Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml
Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl