pAC-Luc 逆病毒载体质粒图谱序列抗性-载体及构建-试剂-生物在线
pAC-Luc 逆病毒载体质粒图谱序列抗性

pAC-Luc 逆病毒载体质粒图谱序列抗性

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产品名称： pAC-Luc 逆病毒载体质粒图谱序列抗性

英文名称： pAC-Luc 逆病毒载体质粒图谱序列抗性说明书价格报价

产品编号： Biovector105802

产品价格： 0

产品产地： Biovector Inc. USA

品牌商标： Biovector, Addgene, ATCC, Invi

更新时间： null

使用范围： null

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  • pAC-Luc 
    (Plasmid #33154)

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    pAC-Luc MCS Luciferase
    Add to Cart
    Available to Academic and Nonprofits Only

    BACKBONE

    • Vector backbone
      pAc5.1/V5-His B
    • Backbone manufacturer
      Invitrogen
    • Backbone size (bp)5400
    • Modifications to backbone
      A PCR product containing the Firefly Luciferase gene and an artificial poly-linker sequence was ligated into pAC5.1/V5-His B between the 5 KpnI and 3 XhoI sites. Primers: Forward: GGATCGGGGTACCTATGGAAGACGCCAAAAACATAAAG Reverse: CACTAGACTCGAGCGTTACAATTTGGACTTTCCGCC Because of the stop codon, V5 and His Tags are not expressed. Restriction sites 3 of Luciferase are Xho1-XbaI-ApaI-BtgI-SacII-V5-MluI-AgeI-6xHIS-PmeI-DraI-StuI-SacI.
    • Vector type
      Insect Expression, Retroviral, Luciferase
    • PromoterActin 5C
    • Selectable markers
      Hygromycin, Blasticidin
    • Tag / Fusion Protein
      • Luciferase (N terminal on backbone)

    GROWTH IN BACTERIA

    • Bacterial Resistance(s)
      Ampicillin
    • Growth Temperature
      37°C
    • Growth Strain(s)
      DH5alpha
    • Copy number
      Unknown

    SEQUENCE INFORMATION

    • Depositor Sequences
      None.
    • Addgene Sequences

    Full plasmid sequence is available only if provided by the depositing laboratory.

    RESOURCE INFORMATION

     
    How to cite this plasmid

    These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

    • For your Materials & Methods section:

      pAC-Luc was a gift from Michael Rosbash (Addgene plasmid # 33154)

    • For your References section:

      Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component. Kadener S, Stoleru D, McDonald M, Nawathean P, Rosbash M. Genes Dev. 2007 Jul 1;21(13):1675-86. Epub 2007 Jun 19.10.1101/gad.1552607 PubMed 17578907