• 靶标种属Mouse
• 经测试应用ICC/IF, IP, Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
• 免疫原

  mouse IgG whole molecule

• 偶联物HRP

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C.
• 存储溶液pH: 7.2
  Preservative: 0.01% Gentamicin sulphate
  Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
• 浓度1 mg 浓度为 2 mg/ml 
  
• 纯度IgG fraction
• 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• 共轭说明Horseradish Peroxidase (HRP)
• 克隆多克隆
• 同种型IgG
• 常规说明The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
• 研究领域
  • Immunology
   
  • Immunoglobulins
   
  • Heavy Chain
   
  • IgG

  • Secondary antibodies
   
  • anti-Mouse
   
  • IgG
   
  • Enzyme
   
  • HRP

应用

Goat Anti-Mouse IgG H&L (HRP) 图像

• 

  Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.
  
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 1 minute

  This image is courtesy of an anonymous Abreview.

  See Abreview

• 

  Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.

  ab49969 Immunoprecipitating HA Tag in human 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)

  IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  The inset negative control image is secondary-only at 1/500 dilution.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789)
  Ab6789 was used at dilution 1/10000 with the primary antibody ab55504 in WB. See the review on ab55504.