Anti-CTNNB1 (S37Y) Mouse Monoclonal Antibody-抗体-抗体-生物在线
Anti-CTNNB1 (S37Y) Mouse Monoclonal Antibody

Anti-CTNNB1 (S37Y) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-CTNNB1 (S37Y) Mouse Monoclonal Antibody

英文名称： Anti-CTNNB1 (S37Y) Mouse Monoclonal Antibody

产品编号： 26296

产品价格： null

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IHC

武汉纽斯特生物技术有限公司
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  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
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  • 所在区域 : 湖北
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  • 邮箱 : neweast3@126.com
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CTNNB1 (S37Y) (26296) 
[100ul]		  

Catalog Number:  26296
Gene Symbol:   CTNNB1
Description:   Anti-CTNNB1 (S37Y) Mouse Monoclonal Antibody
Background:   CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherens junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutant CTNNB1 (ß-catenin) has been implicated in the pathogenesis of several cancers including melanoma, colorectal cancer, hepatocelluar carcinoma, and ovarian cancerMutations has been implicated in the pathogenesis of several cancers.
Immunogen:   A synthetic peptide from the internal region of CTNNB1 which includes the mutation of S37Y, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:2000 
IHC: 1:50-1:100
Concentration:   1mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes S37Y mutant, but not wild-type CTNNB1 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant CTNNB1 (S37Y) and wildtype proteins. Purified His-tagged CTNNB1 (S37Y) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-CTNNB1 (S37Y) monoclonal antibody (Cat. #26296).
Immunofluorescence:  
Immunofluorescence of cells expressing CTNNB1 proteins with anti-CTNNB1 (S37Y) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (WT) plasmid (left column) or pCDNA3-GFP-CTNNB1 (S37Y) plasmid (right column), then fixed and stained with anti-CTNNB1 (S37Y) monoclonal antibody (Cat. #26296).