Anti-EGFR (vIII) Mouse Monoclonal Antibody-抗体-抗体-生物在线
Anti-EGFR (vIII) Mouse Monoclonal Antibody

Anti-EGFR (vIII) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-EGFR (vIII) Mouse Monoclonal Antibody

英文名称： Anti-EGFR (vIII) Mouse Monoclonal Antibody

产品编号： 26176

产品价格： null

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IF、IHC

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
  • 邮编 : 430074
  • 所在区域 : 湖北
  • 电话 : 186****7297
  • 传真 : 027-87561022
  • 邮箱 : neweast3@126.com
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EGFR (vIII) [26176]
[100 µL]		  

Catalog Number:  26176
Gene Symbol:  EGFR/HER1/ERBB1
Description:   Anti-EGFR (vIII) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (NSCLC).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of vIII, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:1000-1:5000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR (vIII), but not wild type EGFR from vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant EGFR (vIII) and wildtype proteins. Purified His-tagged EGFR (vIII) protein (partial, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-EGFR (vIII) mouse monoclonal antibody (Cat. # 26176).
Immunofluorescence:  
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (vIII) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (vIII) plasmid (right column), then fixed and stained with anti-EGFR (vIII) monoclonal antibody (Cat. #26176).
Publications:
1.  Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma
    Tumor Biology. October 2014