人活性ErbB 2 (biotinylated )蛋白片段-蛋白质/抗原/多肽-试剂-生物在线
人活性ErbB 2 (biotinylated )蛋白片段

人活性ErbB 2 (biotinylated )蛋白片段

商家询价

产品名称： 人活性ErbB 2 (biotinylated )蛋白片段

英文名称： Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog C erb B2/neu protein CD340 CD340 antig

产品编号： ab200500

产品价格： 0

产品产地： 英国

品牌商标： abcam

更新时间： null

使用范围： null

深圳市宇德立生物科技有限公司
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进入展台

 

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • AccessionP04626
    • 种属Human
    • 序列TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLS FLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDP LNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDI FHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAG GCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALV TYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEV TAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFG SLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDL SVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNT HLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWG PGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNG SVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGA CQPCPINCTHSCVDLDDKGCPAEQRASPLT
    • 分子量72 kDa including tags
    • 氨基酸23 to 652
    • 标签His tag C-Terminus
    • 额外的序列信息Extracellular domain (AAA75493).
  • 偶联物Biotin

技术指标

Our Abpromise guarantee covers the use of ab200500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Measured by its binding ability in a functional ELISA. Immobilized Herceptin mAb at 3 μg/mL (100 μl/well) can bind ab200500.


    The EC50 of ab200500 is 0.09-1 ng/mL.

  • 应用

    SDS-PAGE

    Functional Studies

  • 内毒素水平< 1.000 Eu/µg
  • 纯度>95% by SDS-PAGE .

  • 形式Lyophilised
  • 补充说明

    The primary amine of ab200500 was chemically labeled by specially optimized long spacer biotin reagent for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes to give superior sensitivity.

  • 浓度由溶解方法决定 

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 95% PBS, 5% Trehalose

    Lyophilised from 0.22 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶It is recommended to reconstitute the lyophilised product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

常规信息

  • 别名
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
    • C erb B2/neu protein
    • CD340
    see all
  • 功能Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    In the nucleus is involved in transcriptional regulation. Associates with the 5-TCAAATTC-3 sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
  • 组织特异性Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
  • 疾病相关Hereditary diffuse gastric cancer
    Glioma
    Ovarian cancer
    Lung cancer
    Gastric cancer
    Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Signaling via SEMA4C promotes phosphorylation at Tyr-1248.
  • 细胞定位Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
  • Information by UniProt

Active human ErbB 2 (biotinylated ) protein fragment 图像

  • SDS-PAGE - Active human ErbB 2 (biotinylated ) protein fragment (ab200500)
    SDS-PAGE - Active human ErbB 2 (biotinylated ) protein fragment (ab200500)

    DTT-reduced SDS-PAGE analysis of ab200500 stained overnight with Coomassie Blue.

    DTT-reduced protein migrates as 90-110 kDa in SDS-PAGE due to glycosylation.