单氨氧化酶A(MAOA)重组蛋白

YB954Ra011
Recombinant Monoamine Oxidase A (MAOA)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 单氨氧化酶A(MAOA)重组蛋白[ PROPERTIES ]
Residues: Gln229~Gly428
Tags: N-terminal His-Tag
Accession: Q64133
Host: E. coli
Subcellular Location: Mitochondrion outer
membrane; Single-pass type IV membrane
protein; Cytoplasmic side.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
 单氨氧化酶A(MAOA)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 6.8
Predicted Molecular Mass: 24.4kDa
Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
 单氨氧化酶A(MAOA)重组蛋白[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8
oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
QI MVLLGDKVKL SSPVTYIDQT DDNIIIETLN HEHYECKYVI SAIPPVLTAK IHFKPELPPE
RNQLIQRLPM GAVIKCMVYY KEAFWKKKDY CGCMIIEDEE APISITLDDT KPDGSMPAIM
GFILARKAER LAKLHKDIRK RKICELYAKV LGSQEALSPV HYEEKNWCEE QYSGGCYTAY
FPPGIMTLYG RVIRQPVG
[ REFERENCES ]
1. Cases O., et al. (1995) Science 268:1763-1766.
2. Popova N.K., et al. (2001) Neurosci. Behav. Physiol. 31:597-602.
3. Ivanova E.A., Popova N.K. (2002) Bull. Exp. Biol. Med. 133:603-605.
4. Naumenko V.S., et al. (2005) Dokl. Biol. Sci. 402:205-207.