pCEP4哺乳动物细胞表达载体质粒图谱序列抗性
产品名称： pCEP4哺乳动物细胞表达载体质粒图谱序列抗性
英文名称： pCEP4哺乳动物细胞表达载体质粒图谱序列抗性
产品编号： Biovector104802
产品价格： 0
产品产地： Biovector Inc. USA
品牌商标： Biovector
更新时间： null
使用范围： null
载体基本信息
|
出品公司: |
Invitrogen |
|
载体名称: |
pCEP4 |
|
质粒类型: |
哺乳动物细胞表达载体 |
|
启动子: |
CMV |
|
表达水平: |
高 |
|
克隆方法: |
多克隆位点，限制性内切酶 |
|
载体大小: |
10410 bp |
|
5 测序引物: |
CMV-F: 5-CGCAAATGGGCGGTAGGCGTG-3 |
|
3 测序引物: |
EBV-R:5-GTGGTTTGTCCAAACTCATC-3 |
|
载体标签: |
/ |
|
载体抗性: |
氨苄 |
|
筛选标记: |
潮霉素 |
|
备注: |
|
|
产品目录号: |
V044-50 |
|
稳定性: |
稳表达 |
|
组成型: |
组成型 |
|
病毒/非病毒: |
非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells. pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
载体序列
LOCUS pCEP4 10410 bp DNA circular SYN
DEFINITION pCEP4
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..10410
/organism="pCEP4"
/mol_type="other DNA"
misc_feature 112..134
/label="pGEX_3_primer"
terminator 279..410
/label="SV40_PA_terminator"
misc_feature 367..386
/label="EBV_rev_primer"
promoter complement(474..555)
/label="CMV_promoter"
misc_feature complement(474..498)
/label="LNCX_primer"
misc_feature complement(481..500)
/label="pCEP_fwd_primer"
misc_feature complement(524..544)
/label="CMV_fwd_primer"
CDS 1305..2045
/label="ORF frame 3"
terminator complement(1566..1836)
/label="TK_PA_terminator"
CDS complement(1844..2869)
/label="ORF frame 3"
gene complement(1847..2854)
/label="hygroB"
/gene="hygroB"
CDS 2111..3481
/label="ORF frame 2"
promoter 2988..3136
/label="TATA_promoter"
gene 3143..3481
/label="tet (852 - 1190)"
/gene="tet (852 - 1190)"
CDS 3385..3996
/label="ORF frame 1"
gene complement(4430..5290)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4430..5290)
/label="ORF frame 3"
promoter complement(5332..5360)
/label="AmpR_promoter"
CDS complement(5914..7128)
/label="ORF frame 1"
CDS 5916..7841
/label="ORF frame 3"
CDS 6031..7143
/label="ORF frame 1"
rep_origin 8145..10315
/label="EBV_origin"
ORIGIN
1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggccttgcc
421 ggcctcgagc ggccgctagc aagcttgcta gcagctggta cccagcttct agagatctga
481 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt
541 tgcgtcaacg gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa
601 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
661 tatccacgcc cattggtgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
721 acgtagatgt actgccaagt aggaaagtcc cgtaaggtca tgtactgggc ataatgccag
781 gcgggccatt taccgtcatt gacgtcaata gggggcggac ttggcatatg atacacttga
841 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
901 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
961 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
1021 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
1081 catggcggtc atattggaca tgagccaata taaatgtaca tattatgata tagatacaac
1141 gtatgcaatg gccaatagcc aatattgatt tatgctatat aaccaatgac taatatggct
TUNEology 波长可调检测卡盒-->