AcuI内切酶-RNAi技术-试剂-生物在线
AcuI内切酶

AcuI内切酶

商家询价

产品名称: AcuI内切酶

英文名称: AcuI

产品编号: HZ2215

产品价格: 0

产品产地: 中国/上海

品牌商标: 沪震生物

更新时间: 2023-08-17T10:24:20

使用范围: null

上海沪震实业有限公司
  • 联系人 : 鲍丽雯
  • 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 139****0749
  • 传真 : 021-60345367
  • 邮箱 : www.shzbio.net

 AcuI内切酶

中文名称:AcuI内切酶
英文名称:AcuI
产品规格:50U
储存条件:冷冻(-20℃)

使用说明

High enzyme concentration may result in star activity.To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml and SAM should be added to a final concentration 0.01mM?Do not use BSA for long incubation.

技术规格
 

Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites CTGAAG(N)16↑
来源 E.coli strain that carries the cloned Acu I gene from Acinetobacter calcoaceticus SRW4
Assayed on Lambda DNA
Optimal buffer Y(33 mM Tris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)+ BSA + SAM
Optimal temperature 37℃
Storage conditions 10 mM Tris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol;Store at -20℃.
Ligations After 2-fold overdigestion with enzyme about 80%of the DNA fragments can be ligated.Of these,80%can be recut.
Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a.of enzyme for 16hoursat 37℃.
Methlation effect Methylation sensitivity not tested
Thermal inactivation enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends Eco57I

 

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