凝胶回收与PCR产物纯化试剂盒-核酸纯化-试剂-生物在线
凝胶回收与PCR产物纯化试剂盒

凝胶回收与PCR产物纯化试剂盒

商家询价

产品名称： 凝胶回收与PCR产物纯化试剂盒

英文名称： NucleoSpin Extract II (250)

产品编号： 740609.250

产品价格： 0

产品产地： 德国

品牌商标： MN(Macherey-Nagel)

更新时间： null

使用范围： null

广州伟杰生物科技有限公司
  • 联系人 :
  • 地址 : 广州市荔湾区西湾路广雅前街2号荔海商务大厦310室
  • 邮编 :
  • 所在区域 : 广东
  • 电话 : 189****9166
  • 传真 :
  • 邮箱 : weijiebio@189.cn
进入展台

PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume

• Two applications in one kit, one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume: 15 μL – highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)

 

   
Technology Silica-membrane technology
Format Mini spin columns
Sample material

< 400 µL PCR reaction mixture
< 400 mg TAE / TBE agarose gel
Fragment size 50 bp–approx. 20 kbp
Typical recovery 70–95%
A260/A280 1.8–1.9
Elution volume 15–30 µL
Preparation time 10 min/6 preps
Binding capacity 25 µg
 
Applications
 

• Purification of PCR products
• Extraction of DNA from agarose gels
• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:
- nucleotides, primers
- enzymes
- mineral oil
- PCR additives (e.g., salts, betaine, DMSO)
- detergents (e.g., Tween 20, Triton X-100)
- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain)

• Support protocol for DNA extraction from polyacrylamide gels
• Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from
   agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)
• Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the
   kit, see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis