确定蛋白中二硫键的数目，位置及二硫键形成的比例。

1). Analytical Disulfide Bond Mapping  

         Analytical disulfide bond mapping is aimed to determine the number, the position and ratio of disulfide bond linkage in a protein.

       We’ll report the ratio and each detected linkage of each system residues in the protein in a project report.

       Due to use of analytical HPLC in this analysis, we require > 0.5mg protein with > 90% purity for S-S mapping. The service is used in the characterization of biologics samples at the development stage and the comparability study.

 2). Micro Disulfide Bond Mapping 

        Micro disulfide bond mapping is often used for research stage protein samples that the disulfide linkage is unknown. The amount of a sample is often limited in a 10ug-100ug range, and the purity of the sample might be low. Therefore, it becomes necessary to use NanoLC-MS/MS system that is of more than thousands fold higher sensitivity in detection of disulfide bond linked peptides.