分子量:104 kDa (single chain), 81 kDa (α subunit) and 23 kDa (β subunit)

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human IGF-I R, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its binding ability in a functional ELISA. 
Immobilized Human IGF-I, Fc Tag (Cat# IG1-H4269) at 5μg/mL (100 l/well),can bind Human IGF-I R, His Tag (Cat# IGR-H5229) with a linear of 0.2-10 g/mL.

产品特性:Human IGF-I R, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 104 kDa (single chain), 81 kDa (α subunit) and 23 kDa (β subunit). The predicted N-terminus is Glu 31. The reducing (R) protein migrates as 120 kDa, 81 kDa and 35 kDa in SDS-PAGE .

产品背景:The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.