SORCS1,液泡蛋白分选受体SORCS抗体

产品编号HZ-11330R
英文名称SORCS1
中文名称液泡蛋白分选受体SORCS抗体
别 名SorCS 1; SORCS; SORCS-1; SORCS receptor 1; Sortilin related VPS10 domain containing receptor 1; VPS10 domain containing receptor SorCS1; VPS10 domain receptor protein SORCS 1; VPS10 domain receptor protein SORCS; VPS10 domain receptor protein SORCS1; VPS10 domain receptor SorCS; FLJ41758; FLJ43475; FLJ44957; hSorCS; OTTHUMP00000058855; SORC1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 细胞类型标志物 Alzheimer's
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
SORCS1,液泡蛋白分选受体SORCS抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量127kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human SORCS1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
SORCS1,液泡蛋白分选受体SORCS抗体PubMedPubMed
产品介绍background:
There are three sorCS genes that have diverse, partially overlapping functions in the central nervous system. In the developing and mature central nervous system, the homologous SorCS1 and SorCS2 genes and the SorCS3 gene are expressed in a combinatorial, non-overlapping pattern. SorCS proteins show homology to the mosaic receptor SorLA and the neurotensin receptor sortilin, based on a common VPS10 domain, which is the hallmark of the SorCS receptor family. SorCS1 is a type 1 receptor containing a VPS10P domain and a leucine-rich domain. Alternative splicing of human SorCS1 results in four isoforms with different cytoplasmic tails and differential expression in tissues. Human SorCS1 is detected in fetal and infant brain and in fetal retina. Alternative splicing of murine SorCS1 also results in four isoforms. Murine isoform 1 is highly expressed in brain and at lower levels in heart, liver and kidney. It is detected in newborn mouse brain and in adult olfactory bulb and cerebral cortex. Murine isoform 2 is highly expressed in liver and at lower levels in heart, brain, kidney and testis.

Function:
SorCS1 belongs to the family of VPS10 domain-containing receptors (the name is derived from the yeast vacuolar protein sorting protein-10, which is involved in sorting of carboxy-peptidase Y from the Golgi apparatus to the vacuole). SorCS1 immunoreactivity is widespread in a population of neurons throughout the brain. Two different types of cellular localization were observed. Most SorCS1 immunoreactive neurons exhibit a punctate cytoplasmic staining which extends into the dendrites, whilst occassionally SorCS1 neuronal immunoreactivity is associated with the plasma membrane.

SORCS1,液泡蛋白分选受体SORCS抗体Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Detected in fetal and infant brain and in fetal retina.

Post-translational modifications:
O-glycosylated.

Similarity:
Belongs to the VPS10-related sortilin family. SORCS subfamily.
Contains 5 BNR repeats.
Contains 1 PKD domain.

Database links:
UniProtKB/Swiss-Prot: Q8WY21.3

SORCS1,液泡蛋白分选受体SORCS抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: brain of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-SORCS1 Polyclonal Antibody, Unconjugated(HZ-11330R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining