信号素3E(SEMA3E)重组蛋白

YB920Mu022
Recombinant Semaphorin 3E (SEMA3E)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 信号素3E(SEMA3E)重组蛋白10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Arg32~Phe516
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: O15041
Host: E. coli
Subcellular Location: Secreted.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 信号素3E(SEMA3E)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.5
Predicted Molecular Mass: 59.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
RLRLSHKEL LNLNRTSIFH SPFGFLDLHT MLLDEYQERL FVGGRDLVYS LSLERISDGY
 信号素3E(SEMA3E)重组蛋白KEIHWPSTAL KMEECIMKGK DAGECANYVR VLHHYNRTHL LTCGTGAFDP VCAFIRVGYH
LEDPLFHLES PRSERGRGRC PFDPSSSFIS TLIGSELFAG LYSDYWSRDA AIFRSMGRLA
HIRTEHDDER LLKEPKFVGS YMIPDNEDRD DNKVYFFFTE KALEAENNAH AIYTRVGRLC
VNDVGGQRIL VNKWSTFLKA RLVCSVPGMN GIDTYFDELE DVFLLPTRDH KNPVIFGLFN
TTSNIFRGHA ICVYHMSSIR AAFNGPYAHK EGPEYHWSVY EGKVPYPRPG SCASKVNGGR
YGTTKDYPDD AIRFARSHPL MYQAIKPAHK KPILVKTDGK YNLKQIAVDR VEAEDGQYDV
LFIGTDNGIV LKVITIYNQE MESMEEVILE ELQIFKDPVP IISMEISSKR QQLYIGSASA
VAQVRF